Detailed information NTG0661350

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMZ77_RS02090
• Genome accession: NZ_CP093014
• Coordinates: 450127..451275 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 445127..456275

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMZ77_RS02065 (MMZ77_02065)	-	446278..446574 (-)	297	WP_031627661.1	DUF167 domain-containing protein	-
MMZ77_RS02070 (MMZ77_02070)	-	446571..447164 (-)	594	WP_003084544.1	YggT family protein	-
MMZ77_RS02075 (MMZ77_02075)	proC	447175..447996 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MMZ77_RS02080 (MMZ77_02080)	-	448008..448700 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMZ77_RS02085 (MMZ77_02085)	pilT	448915..449949 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMZ77_RS02090 (MMZ77_02090)	pilU	450127..451275 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MMZ77_RS02095 (MMZ77_02095)	czcD	451283..452182 (-)	900	WP_003162016.1	CDF family zinc transporter CzcD	-
MMZ77_RS02100 (MMZ77_02100)	-	452336..452740 (+)	405	WP_003084560.1	NINE protein	-
MMZ77_RS02105 (MMZ77_02105)	-	452983..454356 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MMZ77_RS02110 (MMZ77_02110)	-	454353..455537 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=661350 MMZ77_RS02090 WP_003100959.1 450127..451275(+) (pilU) [Pseudomonas aeruginosa strain H17]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661350 MMZ77_RS02090 WP_003100959.1 450127..451275(+) (pilU) [Pseudomonas aeruginosa strain H17]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382