Detailed information NTG0661315

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MM141_RS01990
• Genome accession: NZ_CP093012
• Coordinates: 426206..427354 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421206..432354

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MM141_RS01965 (MM141_01965)	-	422357..422653 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MM141_RS01970 (MM141_01970)	-	422650..423243 (-)	594	WP_003084544.1	YggT family protein	-
MM141_RS01975 (MM141_01975)	proC	423254..424075 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MM141_RS01980 (MM141_01980)	-	424087..424779 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MM141_RS01985 (MM141_01985)	pilT	424994..426028 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MM141_RS01990 (MM141_01990)	pilU	426206..427354 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MM141_RS01995 (MM141_01995)	czcD	427362..428261 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MM141_RS02000 (MM141_02000)	-	428415..428819 (+)	405	WP_003084560.1	NINE protein	-
MM141_RS02005 (MM141_02005)	-	429062..430435 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MM141_RS02010 (MM141_02010)	-	430432..431616 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=661315 MM141_RS01990 WP_003100959.1 426206..427354(+) (pilU) [Pseudomonas aeruginosa strain H20]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661315 MM141_RS01990 WP_003100959.1 426206..427354(+) (pilU) [Pseudomonas aeruginosa strain H20]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382