Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   MME54_RS23745 Genome accession   NZ_CP092918
Coordinates   5271897..5273234 (+) Length   445 a.a.
NCBI ID   WP_054987673.1    Uniprot ID   A0A0N8RM76
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5266897..5278234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS23720 (MME54_23720) pgeF 5267085..5267813 (-) 729 WP_054987669.1 peptidoglycan editing factor PgeF -
  MME54_RS23725 (MME54_23725) rluD 5267810..5268772 (-) 963 WP_054987670.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MME54_RS23730 (MME54_23730) - 5268922..5269944 (+) 1023 WP_054987671.1 outer membrane protein assembly factor BamD -
  MME54_RS23735 (MME54_23735) - 5270073..5270312 (+) 240 WP_054987706.1 PP0621 family protein -
  MME54_RS23740 (MME54_23740) pilS 5270302..5271894 (+) 1593 WP_054987672.1 sensor histidine kinase Regulator
  MME54_RS23745 (MME54_23745) pilR 5271897..5273234 (+) 1338 WP_054987673.1 sigma-54-dependent transcriptional regulator Regulator
  MME54_RS23750 (MME54_23750) thiO 5273363..5274466 (-) 1104 WP_054987674.1 glycine oxidase ThiO -
  MME54_RS23755 (MME54_23755) - 5274494..5274898 (-) 405 WP_054987675.1 type IV pilin protein -
  MME54_RS23760 (MME54_23760) - 5274910..5278116 (-) 3207 WP_054987676.1 pilus assembly protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49749.78 Da        Isoelectric Point: 6.2025

>NTDB_id=661138 MME54_RS23745 WP_054987673.1 5271897..5273234(+) (pilR) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHELLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPQVPVA
MITAHGNLDTAINALKAGAFDFVTKPVDLGRLRELVNSALSLPCAQPARSIDSRLLGDSLAMRTLRNQIGKLARSQAPIY
ISGESGSGKELVARLIHEQGPRIDRPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
LAMQVKLLRAIQEKSIRSVGGQQEQVVDVRILCATHKNLNTEVVEGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAASV
LKRLAGNGAQPAVRLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESASPQEGDGPSLADIDNLED
YLESIERKLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=661138 MME54_RS23745 WP_054987673.1 5271897..5273234(+) (pilR) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGAGCCAACGGCAAAAAATCCTGATAGTCGATGATGAGCCGGATATTCGTGAGCTTCTGGAGATCACGCTGGGGCGCAT
GAAACTCGATACTCGCAGCGCCCGTAACGTTGCCGAGGCGCACGAATTGCTGGCCCGCGAGCCGTTCGACATGTGCCTGA
CCGACATGCGTCTGCCCGACGGTAACGGCCTGGAACTGGTGCAACACATCCAGCACGGCTACCCCCAGGTGCCGGTGGCG
ATGATCACCGCCCACGGCAATCTGGATACCGCAATTAACGCACTCAAGGCCGGGGCTTTCGATTTTGTGACCAAACCGGT
CGACCTCGGCAGGCTGCGCGAGCTGGTCAACAGCGCGCTGAGCCTGCCTTGCGCCCAGCCAGCACGTAGCATCGACAGCC
GTCTGCTGGGCGATTCGCTGGCGATGCGCACCTTGCGCAATCAGATCGGCAAACTGGCGCGCAGTCAGGCCCCCATCTAT
ATCAGCGGCGAGTCGGGCAGCGGCAAGGAGCTTGTCGCACGGTTGATCCACGAACAGGGCCCGCGTATTGACAGGCCGTT
CATACCGGTCAACTGCGGGGCAATTCCTTCCGAACTGATGGAAAGCGAATTCTTTGGTCACCGCAAAGGCAGTTTCAGCG
GTGCCCACGAAGACAAGCCCGGTCTGTTTCAGGCGGCGCATACCGGGACGCTGTTTCTCGACGAGGTCGCCGACCTGCCG
CTGGCGATGCAGGTGAAACTGCTACGAGCCATCCAGGAAAAGTCCATCCGCAGCGTCGGCGGCCAGCAAGAACAGGTCGT
GGACGTGCGCATTCTGTGTGCCACCCACAAGAACCTGAATACCGAGGTTGTAGAAGGTCGCTTCCGTCAGGACCTGTATT
ACCGCCTGAACGTGATCGAAGTGCGCGTGCCCTCATTGCGCGAGCGGCGTGAGGATATCGATCAACTGGCAGCCAGCGTT
CTCAAGCGCCTTGCAGGGAACGGCGCACAACCCGCCGTGCGCCTGAACGCGCAGGCGCTGGAAACGCTGAAGAGCTACCG
CTTCCCCGGTAACGTGCGCGAACTGGAAAACATGCTGGAGCGCGCTTACACGCTTTGTGAAAACGATGAAATCCACGCCA
GCGACCTGCGCCTGACAGAATCCGCCAGCCCTCAGGAGGGCGACGGCCCGAGCCTGGCCGACATCGACAACCTTGAAGAT
TACCTCGAAAGCATCGAGCGCAAGCTCATCCTGCAGGCACTGGAGGAAACCCGCTGGAACCGCACCGCAGCGGCAGAAAG
GCTCAGTTTGTCCTTCAGGTCACTGCGCTACCGGCTGAAGAAGCTGGGCCTGGATTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(136-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)

Response regulator receiver domain (Response_reg)

(7-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N8RM76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

78.604

99.775

0.784
  pilR Acinetobacter baumannii strain A118

48.822

100

0.512
  luxO Vibrio cholerae strain A1552

37.613

99.775

0.375