Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   MME54_RS23740 Genome accession   NZ_CP092918
Coordinates   5270302..5271894 (+) Length   530 a.a.
NCBI ID   WP_054987672.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5265302..5276894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS23720 (MME54_23720) pgeF 5267085..5267813 (-) 729 WP_054987669.1 peptidoglycan editing factor PgeF -
  MME54_RS23725 (MME54_23725) rluD 5267810..5268772 (-) 963 WP_054987670.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MME54_RS23730 (MME54_23730) - 5268922..5269944 (+) 1023 WP_054987671.1 outer membrane protein assembly factor BamD -
  MME54_RS23735 (MME54_23735) - 5270073..5270312 (+) 240 WP_054987706.1 PP0621 family protein -
  MME54_RS23740 (MME54_23740) pilS 5270302..5271894 (+) 1593 WP_054987672.1 sensor histidine kinase Regulator
  MME54_RS23745 (MME54_23745) pilR 5271897..5273234 (+) 1338 WP_054987673.1 sigma-54-dependent transcriptional regulator Regulator
  MME54_RS23750 (MME54_23750) thiO 5273363..5274466 (-) 1104 WP_054987674.1 glycine oxidase ThiO -
  MME54_RS23755 (MME54_23755) - 5274494..5274898 (-) 405 WP_054987675.1 type IV pilin protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58297.30 Da        Isoelectric Point: 6.7804

>NTDB_id=661137 MME54_RS23740 WP_054987672.1 5270302..5271894(+) (pilS) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MTADTPSLASPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQLF
SLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEELNALILQRMRTGILVLDAHRQVLLANQGALTLLGHERLVGQIID
AYSPQLVERLRQWLVNPIMVPRSITTSPIGPVLQPSFVALNRGDQRQTLIFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSTEDRRLGQIIHEQSGRIDQVIENVLQLSRRREAQPELLDLKTWLEQFVTEFRSAAAAN
QSIHIDISPGTLTTRMDSGQLTQIMTNLLQNGLRYSAKHHPSAQVWLKLHHDWKSDLPILEVLDDGPGVSEQHLSKMFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYVPRQGGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=661137 MME54_RS23740 WP_054987672.1 5270302..5271894(+) (pilS) [Pseudomonas syringae pv. helianthi strain LMG 5067]
GTGACCGCTGATACGCCCTCCCTTGCCAGCCCGCAGGCGCAGCGGATTCTGCGGCTGTACCACCTTTACCGACTGACCAT
TGGCGTCGTACTGGTCTTGCTGATCTCCAGCAGCCTGGAAACCGAACTGCTGCAACTGGCCAACCCGAATCTGTTCCGTT
CGGGCTGCTGGTTGTATCTGGTGTTCAACATTCTGGTGGTGGTGCTGCTGGAACGCCCGAGCCGTCAGGCACAGCTGTTC
AGCCTGGCAATGACCGACGCAATCATGCTTTCCGCGCTGTTCTACGCAGCGGGCGGCCCTTCCAGCGGCATCGGCAATCT
GCTGATTGCTTCAGTGGCGATCAGCAATGTGCTGCTGCGTGGCCGTATCGGTCTGCTGATCGCCGCTGTTTCTGCCATTG
GCATCATCTACCTGACGTTCTACATCAGCTTCGGCACACCGTCGGTCTCCAATGACTACGTGCAGGCCGGCTCGCTGGGT
GCCCTGTGCTTCGCCGCAGCATTGCTGGTGCAGGCTCTGACGGCACGCATGCAGGTCAGTGAAGTGCTGGCCGAACAGCG
CGCTGCGGATGTCGACAGCCTTGAAGAACTCAACGCACTGATCCTGCAACGCATGCGCACCGGGATTCTGGTACTGGATG
CCCACCGCCAGGTGTTGCTGGCCAATCAGGGGGCCCTGACCCTGCTGGGCCACGAGCGACTGGTCGGGCAGATTATCGAC
GCCTACTCGCCGCAACTGGTAGAACGTCTGCGCCAGTGGCTCGTCAATCCGATCATGGTGCCGCGCAGCATCACGACTTC
CCCCATCGGGCCCGTGCTGCAACCGAGCTTTGTCGCACTCAATCGTGGTGATCAACGCCAGACGCTGATCTTTCTCGAAG
ACCTGTCGCAGATTGCCCATCAGGCGCAGCAGCTCAAACTCGCCGCACTGGGACGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCGGTCAGTCATGCTGCACAACTGCTCAACGAGTCGGAAGAACTCAGCACGGAAGATAGACG
CCTCGGGCAGATCATCCATGAGCAGTCTGGACGAATTGATCAGGTCATTGAAAACGTTCTGCAACTGTCACGTCGGCGCG
AGGCGCAACCCGAACTGCTTGATCTGAAAACCTGGCTGGAACAGTTTGTGACCGAGTTTCGCAGCGCCGCCGCGGCCAAT
CAGTCGATTCATATCGACATCAGCCCCGGCACGCTGACGACGCGCATGGATTCTGGACAACTGACCCAAATCATGACCAA
CCTGTTACAGAACGGCCTGCGCTACAGCGCCAAACATCATCCATCCGCGCAGGTCTGGCTGAAGCTGCATCACGACTGGA
AAAGCGATTTGCCCATCCTGGAAGTGCTGGACGACGGGCCGGGGGTCAGCGAACAACACCTGAGCAAGATGTTCGAGCCG
TTTTTCACTACAGAGAGCAAAGGCACCGGTCTTGGTCTATATCTGTCACGCGAGCTGTGCGAAAGCAATCAGGCGCACCT
GGAATATGTCCCGCGACAAGGTGGCGGCAGTTGCTTGCGCATCACCTTCGCCCACCCGTTCAAAACGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.205

100

0.613