Detailed information NTG0660765

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMB16_RS02035
• Genome accession: NZ_CP092846
• Coordinates: 439084..440232 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ISS SRV-K
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434084..445232

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMB16_RS02010 (MMB16_02010)	-	435235..435531 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MMB16_RS02015 (MMB16_02015)	-	435528..436121 (-)	594	WP_003084544.1	YggT family protein	-
MMB16_RS02020 (MMB16_02020)	proC	436132..436953 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MMB16_RS02025 (MMB16_02025)	-	436965..437657 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMB16_RS02030 (MMB16_02030)	pilT	437872..438906 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMB16_RS02035 (MMB16_02035)	pilU	439084..440232 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MMB16_RS02040 (MMB16_02040)	czcD	440240..441139 (-)	900	WP_003118804.1	CDF family zinc transporter CzcD	-
MMB16_RS02045 (MMB16_02045)	-	441293..441697 (+)	405	WP_003084560.1	NINE protein	-
MMB16_RS02050 (MMB16_02050)	-	441940..443313 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MMB16_RS02055 (MMB16_02055)	-	443310..444493 (+)	1184	Protein_409	PLP-dependent aspartate aminotransferase family protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=660765 MMB16_RS02035 WP_003100959.1 439084..440232(+) (pilU) [Pseudomonas aeruginosa strain ISS SRV-K]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=660765 MMB16_RS02035 WP_003100959.1 439084..440232(+) (pilU) [Pseudomonas aeruginosa strain ISS SRV-K]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGTATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGTAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382