Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MK848_RS10355 Genome accession   NZ_CP092824
Coordinates   2026491..2027882 (-) Length   463 a.a.
NCBI ID   WP_101172232.1    Uniprot ID   -
Organism   Bacillus subtilis strain S1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2021491..2032882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS10320 (MK848_10315) flgL 2021663..2022559 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  MK848_RS10325 (MK848_10320) flgK 2022570..2024093 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  MK848_RS10330 (MK848_10325) flgN 2024112..2024594 (-) 483 WP_014478127.1 flagellar protein FlgN -
  MK848_RS10335 (MK848_10330) flgM 2024610..2024876 (-) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  MK848_RS10340 (MK848_10335) yvyF 2024957..2025376 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  MK848_RS10345 (MK848_10340) comFC 2025449..2026171 (-) 723 WP_033884179.1 comF operon protein ComFC Machinery gene
  MK848_RS10350 (MK848_10345) comFB 2026135..2026431 (-) 297 WP_014478130.1 late competence protein ComFB -
  MK848_RS10355 (MK848_10350) comFA 2026491..2027882 (-) 1392 WP_101172232.1 ATP-dependent helicase ComFA Machinery gene
  MK848_RS10360 (MK848_10355) fakBA 2027988..2028833 (-) 846 WP_003244125.1 DegV family protein -
  MK848_RS10365 (MK848_10360) degU 2028931..2029620 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  MK848_RS10370 (MK848_10365) degS 2029703..2030860 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  MK848_RS10375 (MK848_10370) yvyE 2031077..2031730 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52655.85 Da        Isoelectric Point: 10.1764

>NTDB_id=660435 MK848_RS10355 WP_101172232.1 2026491..2027882(-) (comFA) [Bacillus subtilis strain S1]
MNVPVEKNSSFSKELQQRLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGAEISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIKFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=660435 MK848_RS10355 WP_101172232.1 2026491..2027882(-) (comFA) [Bacillus subtilis strain S1]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGAGGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGAAATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCATTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAAAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGGGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.704

100

0.987