Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MK848_RS04625 Genome accession   NZ_CP092824
Coordinates   943575..944645 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain S1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 938575..949645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS04580 (MK848_04585) tapA 939054..939815 (-) 762 WP_015251716.1 amyloid fiber anchoring/assembly protein TapA -
  MK848_RS04585 (MK848_04590) yqzG 940087..940413 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  MK848_RS04590 (MK848_04595) spoIITA 940455..940634 (-) 180 WP_003230176.1 YqzE family protein -
  MK848_RS04595 (MK848_04600) comGG 940705..941079 (-) 375 WP_015251714.1 ComG operon protein ComGG Machinery gene
  MK848_RS04600 (MK848_04605) comGF 941080..941463 (-) 384 WP_015251713.1 ComG operon protein ComGF Machinery gene
  MK848_RS04605 (MK848_04610) comGE 941489..941836 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  MK848_RS04610 (MK848_04615) comGD 941820..942251 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  MK848_RS04615 (MK848_04620) comGC 942241..942537 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  MK848_RS04620 (MK848_04625) comGB 942551..943588 (-) 1038 WP_101172386.1 comG operon protein ComGB Machinery gene
  MK848_RS04625 (MK848_04630) comGA 943575..944645 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  MK848_RS04630 (MK848_04635) - 944858..945055 (-) 198 WP_029726717.1 hypothetical protein -
  MK848_RS04635 (MK848_04640) corA 945057..946010 (-) 954 WP_004399136.1 magnesium transporter CorA -
  MK848_RS04640 (MK848_04645) yqhB 946153..947481 (+) 1329 WP_029726716.1 hemolysin family protein -
  MK848_RS04645 (MK848_04650) rsbRD 947534..948370 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  MK848_RS04655 (MK848_04660) mgsR 948594..948974 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  MK848_RS04660 (MK848_04665) yqgY 949206..949451 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=660415 MK848_RS04625 WP_004399124.1 943575..944645(-) (comGA) [Bacillus subtilis strain S1]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=660415 MK848_RS04625 WP_004399124.1 943575..944645(-) (comGA) [Bacillus subtilis strain S1]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1