Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MKF36_RS16530 Genome accession   NZ_CP092750
Coordinates   3355152..3356537 (-) Length   461 a.a.
NCBI ID   WP_042635531.1    Uniprot ID   -
Organism   Bacillus velezensis strain KS04AU     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3350152..3361537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF36_RS16495 (MKF36_16495) flgL 3350327..3351244 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  MKF36_RS16500 (MKF36_16500) flgK 3351256..3352773 (-) 1518 WP_007407478.1 flagellar hook-associated protein FlgK -
  MKF36_RS16505 (MKF36_16505) - 3352789..3353271 (-) 483 WP_003151394.1 flagellar protein FlgN -
  MKF36_RS16510 (MKF36_16510) flgM 3353286..3353552 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  MKF36_RS16515 (MKF36_16515) - 3353622..3354041 (-) 420 WP_042635530.1 TIGR03826 family flagellar region protein -
  MKF36_RS16520 (MKF36_16520) comFC 3354115..3354804 (-) 690 WP_197061611.1 phosphoribosyltransferase family protein Machinery gene
  MKF36_RS16525 (MKF36_16525) - 3354810..3355094 (-) 285 WP_007407481.1 competence protein ComFB -
  MKF36_RS16530 (MKF36_16530) comFA 3355152..3356537 (-) 1386 WP_042635531.1 DEAD/DEAH box helicase Machinery gene
  MKF36_RS16535 (MKF36_16535) - 3356646..3357494 (-) 849 WP_007407483.1 DegV family protein -
  MKF36_RS16540 (MKF36_16540) degU 3357592..3358281 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  MKF36_RS16545 (MKF36_16545) degS 3358358..3359521 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  MKF36_RS16550 (MKF36_16550) - 3359744..3360391 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52634.00 Da        Isoelectric Point: 10.2284

>NTDB_id=660051 MKF36_RS16530 WP_042635531.1 3355152..3356537(-) (comFA) [Bacillus velezensis strain KS04AU]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVVMGRADELTSLYSWNQAHENSWENVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=660051 MKF36_RS16530 WP_042635531.1 3355152..3356537(-) (comFA) [Bacillus velezensis strain KS04AU]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGTATTAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAATCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCGTGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCATGAAAACAG
CTGGGAGAATGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACCCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGAAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACCGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTTCATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623