NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	MKF36_RS11780	Genome accession	NZ_CP092750
Coordinates	2410144..2411214 (-)	Length	356 a.a.
NCBI ID	WP_012117985.1	Uniprot ID	A0A411A7V3
Organism	Bacillus velezensis strain KS04AU
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2405144..2416214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKF36_RS11730 (MKF36_11730)	-	2405204..2405788 (-)	585	WP_007408328.1	signal peptidase I	-
MKF36_RS11735 (MKF36_11735)	tapA	2405760..2406431 (-)	672	WP_042635356.1	amyloid fiber anchoring/assembly protein TapA	-
MKF36_RS11740 (MKF36_11740)	-	2406690..2407019 (+)	330	WP_020954231.1	DUF3889 domain-containing protein	-
MKF36_RS11745 (MKF36_11745)	-	2407059..2407238 (-)	180	WP_003153093.1	YqzE family protein	-
MKF36_RS11750 (MKF36_11750)	comGG	2407295..2407672 (-)	378	WP_032866434.1	competence type IV pilus minor pilin ComGG	Machinery gene
MKF36_RS11755 (MKF36_11755)	comGF	2407673..2408173 (-)	501	WP_262982688.1	competence type IV pilus minor pilin ComGF	-
MKF36_RS11760 (MKF36_11760)	comGE	2408082..2408396 (-)	315	WP_007408323.1	competence type IV pilus minor pilin ComGE	-
MKF36_RS11765 (MKF36_11765)	comGD	2408380..2408817 (-)	438	WP_012117983.1	competence type IV pilus minor pilin ComGD	Machinery gene
MKF36_RS11770 (MKF36_11770)	comGC	2408807..2409073 (-)	267	WP_042635730.1	competence type IV pilus major pilin ComGC	Machinery gene
MKF36_RS11775 (MKF36_11775)	comGB	2409120..2410157 (-)	1038	WP_032866436.1	competence type IV pilus assembly protein ComGB	Machinery gene
MKF36_RS11780 (MKF36_11780)	comGA	2410144..2411214 (-)	1071	WP_012117985.1	competence type IV pilus ATPase ComGA	Machinery gene
MKF36_RS11785 (MKF36_11785)	-	2411406..2412356 (-)	951	WP_032870601.1	magnesium transporter CorA family protein	-
MKF36_RS11790 (MKF36_11790)	-	2412502..2413803 (+)	1302	WP_012117986.1	hemolysin family protein	-
MKF36_RS11795 (MKF36_11795)	-	2413841..2414671 (-)	831	WP_007408317.1	STAS domain-containing protein	-
MKF36_RS11805 (MKF36_11805)	-	2414878..2415258 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
MKF36_RS11810 (MKF36_11810)	-	2415495..2415740 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40557.86 Da Isoelectric Point: 9.4784

                                >NTDB_id=660032 MKF36_RS11780 WP_012117985.1 2410144..2411214(-) (comGA) [Bacillus velezensis strain KS04AU]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=660032 MKF36_RS11780 WP_012117985.1 2410144..2411214(-) (comGA) [Bacillus velezensis strain KS04AU]

GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0660032