Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   MK801_RS00435 Genome accession   NZ_CP092748
Coordinates   80173..80673 (+) Length   166 a.a.
NCBI ID   WP_047207005.1    Uniprot ID   A0A443L609
Organism   Lactococcus lactis strain 17M1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 61580..84611 80173..80673 within 0


Gene organization within MGE regions


Location: 61580..84611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK801_RS00320 (MK801_00320) - 61580..61891 (-) 312 WP_047206785.1 sigma factor-like helix-turn-helix DNA-binding protein -
  MK801_RS00325 (MK801_00325) - 61996..62343 (-) 348 WP_254255583.1 hypothetical protein -
  MK801_RS00330 (MK801_00330) - 62417..62917 (-) 501 WP_254255584.1 hypothetical protein -
  MK801_RS00335 (MK801_00335) - 63038..64195 (+) 1158 Protein_55 IS3 family transposase -
  MK801_RS12020 - 64348..64546 (-) 199 Protein_56 hypothetical protein -
  MK801_RS00345 (MK801_00345) - 64550..64744 (-) 195 WP_058224627.1 DUF1655 domain-containing protein -
  MK801_RS00350 (MK801_00350) - 64824..65525 (-) 702 WP_254255586.1 Rha family transcriptional regulator -
  MK801_RS00355 (MK801_00355) - 65683..65877 (-) 195 WP_057720918.1 hypothetical protein -
  MK801_RS00360 (MK801_00360) - 66029..66625 (+) 597 WP_240664918.1 helix-turn-helix transcriptional regulator -
  MK801_RS00365 (MK801_00365) - 66743..67111 (+) 369 WP_012898676.1 TIGR02328 family protein -
  MK801_RS00370 (MK801_00370) - 67995..68321 (+) 327 WP_058208551.1 hypothetical protein -
  MK801_RS00375 (MK801_00375) - 68678..68872 (+) 195 WP_012898673.1 hypothetical protein -
  MK801_RS00380 (MK801_00380) - 69189..69422 (+) 234 WP_057720923.1 hypothetical protein -
  MK801_RS00385 (MK801_00385) - 70506..70670 (+) 165 WP_254255587.1 bacteriocin -
  MK801_RS00390 (MK801_00390) - 71116..72300 (+) 1185 WP_254255588.1 tyrosine-type recombinase/integrase -
  MK801_RS00395 (MK801_00395) - 72590..73036 (+) 447 WP_003131961.1 hypothetical protein -
  MK801_RS00400 (MK801_00400) - 73124..73396 (-) 273 WP_003131960.1 chorismate mutase -
  MK801_RS00405 (MK801_00405) - 73914..74402 (+) 489 WP_003131959.1 GAF domain-containing protein -
  MK801_RS00410 (MK801_00410) - 74433..75074 (+) 642 WP_057720925.1 phosphotransferase -
  MK801_RS00415 (MK801_00415) dnaX 75123..76781 (+) 1659 WP_057720926.1 DNA polymerase III subunit gamma/tau -
  MK801_RS00420 (MK801_00420) - 76815..78281 (-) 1467 WP_017865200.1 amino acid permease -
  MK801_RS00425 (MK801_00425) - 78804..79658 (+) 855 WP_012898664.1 undecaprenyl-diphosphate phosphatase -
  MK801_RS00430 (MK801_00430) rpsF 79850..80143 (+) 294 WP_003131954.1 30S ribosomal protein S6 -
  MK801_RS00435 (MK801_00435) ssb 80173..80673 (+) 501 WP_047207005.1 single-stranded DNA-binding protein Machinery gene
  MK801_RS00440 (MK801_00440) rpsR 80847..81092 (+) 246 WP_003131952.1 30S ribosomal protein S18 -
  MK801_RS00445 (MK801_00445) - 81292..82065 (+) 774 WP_254255589.1 metallophosphoesterase -
  MK801_RS00450 (MK801_00450) - 82147..82455 (-) 309 WP_010906371.1 DUF960 domain-containing protein -
  MK801_RS00455 (MK801_00455) - 82493..83341 (-) 849 WP_057720928.1 MurR/RpiR family transcriptional regulator -
  MK801_RS00460 (MK801_00460) gnd 83706..84611 (+) 906 WP_057720931.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -

Sequence


Protein


Download         Length: 166 a.a.        Molecular weight: 18274.10 Da        Isoelectric Point: 5.2279

>NTDB_id=659943 MK801_RS00435 WP_047207005.1 80173..80673(+) (ssb) [Lactococcus lactis strain 17M1]
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGASAGSYSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEIS
DDDLPF

Nucleotide


Download         Length: 501 bp        

>NTDB_id=659943 MK801_RS00435 WP_047207005.1 80173..80673(+) (ssb) [Lactococcus lactis strain 17M1]
ATGATTAACAATGTTGTATTAGTAGGACGCATTACTCGCGATCCTGAACTTCGATACACCCCTCAAAATCAAGCTGTCGC
AACATTTTCATTGGCTGTAAACCGTCAATTTAAAAATGCGAATGGCGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGCCAACAAGCTGAGAATTTGGCGAATTGGGCTAAAAAAGGAGCTTTGATTGGGGTAACTGGTCGAATTCAAACACGT
AACTATGAAAATCAACAAGGTCAACGCGTTTATGTGACTGAGGTTGTGGCTGACAGTTTCCAAATGTTGGAAAGTAGATC
TGCTCGTGAAGGTATGGGAGGCGGAGCTTCTGCTGGTTCATATTCTGCACCTAGCCAATCTACAAATAATACTCCACGTC
CACAAACGAATAACAATAATGCAACACCGAATTTCGGTCGTGATGCTGATCCGTTTGGTAGCTCACCGATGGAAATTTCG
GATGATGACCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A443L609

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60.465

100

0.627

  ssbA Bacillus subtilis subsp. subtilis str. 168

57.803

100

0.602

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

63.855

0.373

  ssb Glaesserella parasuis strain SC1401

35.028

100

0.373