Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AVFI_RS12035 Genome accession   NZ_CP092712
Coordinates   2632259..2632837 (-) Length   192 a.a.
NCBI ID   WP_005421098.1    Uniprot ID   A0AAV3ER53
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2627259..2637837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS12015 (AVFI_12015) aroB 2628105..2629193 (-) 1089 WP_011262699.1 3-dehydroquinate synthase -
  AVFI_RS12020 (AVFI_12020) aroK 2629230..2629748 (-) 519 WP_005421091.1 shikimate kinase AroK -
  AVFI_RS12025 (AVFI_12025) pilQ 2629973..2631661 (-) 1689 WP_012533907.1 type IV pilus secretin PilQ Machinery gene
  AVFI_RS12030 (AVFI_12030) pilP 2631748..2632266 (-) 519 WP_005421095.1 pilus assembly protein PilP Machinery gene
  AVFI_RS12035 (AVFI_12035) pilO 2632259..2632837 (-) 579 WP_005421098.1 type 4a pilus biogenesis protein PilO Machinery gene
  AVFI_RS12040 (AVFI_12040) - 2632837..2633403 (-) 567 WP_011262703.1 PilN domain-containing protein -
  AVFI_RS12045 (AVFI_12045) pilM 2633388..2634389 (-) 1002 WP_054775331.1 type IV pilus biogenesis protein PilM -
  AVFI_RS12050 (AVFI_12050) - 2634528..2637026 (+) 2499 WP_026029395.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22463.87 Da        Isoelectric Point: 4.8892

>NTDB_id=659798 AVFI_RS12035 WP_005421098.1 2632259..2632837(-) (pilO) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MDWRELDLDEITEWPIVPQLLVILLLCLCIQGAGYWFWLQPIQESIEQSKLDEISLKTTVKYKYNQVAAMPKMKDQLNEL
NLRYEFLSQQLPAQKELASMLSGINQVGIQNKLTFTRIDWGEKQNKTFLYKLPLNIELTGQYHDIGSFSEAMADLPRIVS
LENIDIQRVSKESSTLHFRVMAYTYQFRGDHE

Nucleotide


Download         Length: 579 bp        

>NTDB_id=659798 AVFI_RS12035 WP_005421098.1 2632259..2632837(-) (pilO) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGGATTGGCGCGAATTAGATTTAGATGAAATTACGGAATGGCCGATTGTTCCGCAGCTGTTAGTGATTTTGTTGTTGTG
TTTATGTATTCAAGGTGCTGGATACTGGTTTTGGTTACAACCAATACAAGAAAGTATCGAGCAGTCAAAATTGGATGAAA
TAAGCTTAAAAACAACGGTGAAATATAAATATAACCAAGTTGCTGCTATGCCAAAAATGAAAGATCAATTGAATGAATTG
AATCTACGATATGAGTTTTTGTCGCAGCAGTTACCCGCTCAAAAAGAGTTAGCAAGCATGCTTTCAGGTATCAACCAGGT
TGGTATTCAAAATAAGCTTACTTTTACTCGGATTGACTGGGGTGAGAAGCAAAATAAAACCTTTTTATATAAGTTACCTC
TGAATATTGAGCTGACAGGGCAATACCACGACATCGGGTCGTTTTCTGAAGCCATGGCTGATTTACCACGTATTGTCAGC
TTGGAAAATATTGATATTCAAAGGGTGAGTAAGGAGAGTAGTACATTGCACTTTCGTGTAATGGCTTATACCTACCAATT
TAGGGGGGACCATGAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

62.162

96.354

0.599
  pilO Vibrio campbellii strain DS40M4

57.979

97.917

0.568