Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   MKF35_RS12290 Genome accession   NZ_CP092629
Coordinates   2659370..2660707 (-) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain HU20     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2657586..2681521 2659370..2660707 within 0


Gene organization within MGE regions


Location: 2657586..2681521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS12280 - 2657586..2658095 (-) 510 WP_240282914.1 Tfp pilus assembly protein FimT/FimU -
  MKF35_RS12285 thiO 2658230..2659324 (+) 1095 WP_031801325.1 glycine oxidase ThiO -
  MKF35_RS12290 pilR 2659370..2660707 (-) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  MKF35_RS12295 pilS 2660722..2662314 (-) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  MKF35_RS12300 - 2662304..2662549 (-) 246 WP_003102591.1 PP0621 family protein -
  MKF35_RS12305 - 2662706..2663731 (-) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  MKF35_RS12310 rluD 2663877..2664839 (+) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MKF35_RS12315 pgeF 2664836..2665564 (+) 729 WP_003123393.1 peptidoglycan editing factor PgeF -
  MKF35_RS12320 clpB 2665720..2668284 (+) 2565 WP_047106652.1 ATP-dependent chaperone ClpB -
  MKF35_RS12340 - 2668923..2670085 (+) 1163 WP_086937300.1 IS3-like element IS222 family transposase -
  MKF35_RS12345 tpsA4 2670235..2674488 (-) 4254 WP_034000898.1 two-partner secretion system exoprotein TpsA4 -
  MKF35_RS12350 - 2674537..2676174 (-) 1638 WP_010895677.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  MKF35_RS12355 zapE 2676723..2677814 (+) 1092 WP_010895676.1 cell division protein ZapE -
  MKF35_RS12360 - 2677854..2679161 (-) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -
  MKF35_RS12365 - 2679225..2679395 (-) 171 WP_003094672.1 DUF3094 family protein -
  MKF35_RS12370 - 2679421..2679870 (-) 450 WP_003161439.1 MOSC domain-containing protein -
  MKF35_RS12375 - 2679867..2680496 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MKF35_RS12380 - 2680629..2681054 (+) 426 WP_003116252.1 GNAT family N-acetyltransferase -
  MKF35_RS12385 - 2681051..2681521 (+) 471 WP_003094664.1 hypothetical protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=659280 MKF35_RS12290 WP_003094694.1 2659370..2660707(-) (pilR) [Pseudomonas aeruginosa strain HU20]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=659280 MKF35_RS12290 WP_003094694.1 2659370..2660707(-) (pilR) [Pseudomonas aeruginosa strain HU20]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACTCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAAAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384