Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   MKF35_RS12295 Genome accession   NZ_CP092629
Coordinates   2660722..2662314 (-) Length   530 a.a.
NCBI ID   WP_003123394.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HU20     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2657586..2681521 2660722..2662314 within 0


Gene organization within MGE regions


Location: 2657586..2681521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS12280 - 2657586..2658095 (-) 510 WP_240282914.1 Tfp pilus assembly protein FimT/FimU -
  MKF35_RS12285 thiO 2658230..2659324 (+) 1095 WP_031801325.1 glycine oxidase ThiO -
  MKF35_RS12290 pilR 2659370..2660707 (-) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  MKF35_RS12295 pilS 2660722..2662314 (-) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  MKF35_RS12300 - 2662304..2662549 (-) 246 WP_003102591.1 PP0621 family protein -
  MKF35_RS12305 - 2662706..2663731 (-) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  MKF35_RS12310 rluD 2663877..2664839 (+) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MKF35_RS12315 pgeF 2664836..2665564 (+) 729 WP_003123393.1 peptidoglycan editing factor PgeF -
  MKF35_RS12320 clpB 2665720..2668284 (+) 2565 WP_047106652.1 ATP-dependent chaperone ClpB -
  MKF35_RS12340 - 2668923..2670085 (+) 1163 WP_086937300.1 IS3-like element IS222 family transposase -
  MKF35_RS12345 tpsA4 2670235..2674488 (-) 4254 WP_034000898.1 two-partner secretion system exoprotein TpsA4 -
  MKF35_RS12350 - 2674537..2676174 (-) 1638 WP_010895677.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  MKF35_RS12355 zapE 2676723..2677814 (+) 1092 WP_010895676.1 cell division protein ZapE -
  MKF35_RS12360 - 2677854..2679161 (-) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -
  MKF35_RS12365 - 2679225..2679395 (-) 171 WP_003094672.1 DUF3094 family protein -
  MKF35_RS12370 - 2679421..2679870 (-) 450 WP_003161439.1 MOSC domain-containing protein -
  MKF35_RS12375 - 2679867..2680496 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MKF35_RS12380 - 2680629..2681054 (+) 426 WP_003116252.1 GNAT family N-acetyltransferase -
  MKF35_RS12385 - 2681051..2681521 (+) 471 WP_003094664.1 hypothetical protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58999.18 Da        Isoelectric Point: 6.6886

>NTDB_id=659281 MKF35_RS12295 WP_003123394.1 2660722..2662314(-) (pilS) [Pseudomonas aeruginosa strain HU20]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEIIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=659281 MKF35_RS12295 WP_003123394.1 2660722..2662314(-) (pilS) [Pseudomonas aeruginosa strain HU20]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTACTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGTCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTGGGC
CGCCACAGTCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAATCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.811

100

0.998