Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MKF35_RS09665 Genome accession   NZ_CP092629
Coordinates   2075776..2076840 (+) Length   354 a.a.
NCBI ID   WP_047105348.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070776..2081840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS09655 - 2071747..2073015 (+) 1269 WP_004347579.1 malic enzyme-like NAD(P)-binding protein -
  MKF35_RS09660 - 2073120..2075591 (-) 2472 WP_017148635.1 penicillin-binding protein 1A -
  MKF35_RS09665 pilM 2075776..2076840 (+) 1065 WP_047105348.1 type IV pilus assembly protein PilM Machinery gene
  MKF35_RS09670 pilN 2076840..2077436 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  MKF35_RS09675 pilO 2077433..2078056 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  MKF35_RS09680 pilP 2078053..2078577 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  MKF35_RS09685 pilQ 2078631..2080775 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  MKF35_RS09690 aroK 2080787..2081305 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37997.51 Da        Isoelectric Point: 4.5446

>NTDB_id=659276 MKF35_RS09665 WP_047105348.1 2075776..2076840(+) (pilM) [Pseudomonas aeruginosa strain HU20]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQELDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=659276 MKF35_RS09665 WP_047105348.1 2075776..2076840(+) (pilM) [Pseudomonas aeruginosa strain HU20]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGAGCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466