Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MI302_RS09790 Genome accession   NZ_CP092445
Coordinates   1714563..1716827 (+) Length   754 a.a.
NCBI ID   WP_239725643.1    Uniprot ID   -
Organism   Thermus sp. NEB1569     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709563..1721827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS09765 (MI302_09765) - 1710046..1711050 (+) 1005 WP_054392201.1 homoisocitrate dehydrogenase -
  MI302_RS09770 (MI302_09770) pilM 1711230..1712363 (+) 1134 WP_239725640.1 type IV pilus assembly protein PilM Machinery gene
  MI302_RS09775 (MI302_09775) pilN 1712356..1712979 (+) 624 WP_239725641.1 flagellar protein FliT Machinery gene
  MI302_RS09780 (MI302_09780) pilO 1712969..1713592 (+) 624 WP_015717936.1 type 4a pilus biogenesis protein PilO Machinery gene
  MI302_RS09785 (MI302_09785) pilW 1713589..1714551 (+) 963 WP_239725642.1 competence protein Machinery gene
  MI302_RS09790 (MI302_09790) pilQ 1714563..1716827 (+) 2265 WP_239725643.1 secretin N-terminal domain-containing protein Machinery gene
  MI302_RS09795 (MI302_09795) aroC 1716931..1718082 (+) 1152 WP_019550802.1 chorismate synthase -
  MI302_RS09800 (MI302_09800) - 1718100..1718654 (+) 555 WP_015717940.1 shikimate kinase -
  MI302_RS09805 (MI302_09805) - 1718632..1719687 (+) 1056 WP_239725644.1 3-dehydroquinate synthase family protein -
  MI302_RS09810 (MI302_09810) rsmF 1719742..1721109 (-) 1368 WP_239725645.1 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF -
  MI302_RS12135 - 1721167..1721295 (+) 129 WP_255721633.1 hypothetical protein -
  MI302_RS09815 (MI302_09815) acpS 1721292..1721669 (+) 378 WP_126170861.1 holo-ACP synthase -

Sequence

Protein


Download         Length: 754 a.a.        Molecular weight: 82520.77 Da        Isoelectric Point: 5.4264

>NTDB_id=657533 MI302_RS09790 WP_239725643.1 1714563..1716827(+) (pilQ) [Thermus sp. NEB1569]
MKLLIPTLLALGAGAWAGSLPQEPRFDAKVDLKVSESQVRAGLTLPLDVVLEALARSVGLQPLIYRAYDASGDPAKAQPP
LPNIKLDFQGKPFREVWDLLFATYGTQFNLDYLFLPPDVVVVAPTQVITALVDAPSRTGAMERKPYLVAIPEIAYRRTET
DAQGQARTVVNIDGAKAWVQNDLLPFLSREASGLSVNWIVVEEGGKLRALLSVLATPEQHTRFSDILQRAGIDFRPLPAL
TLPKPRVERTYTLTHATFPELLAFLQGQVPNAQIAVVPTDPKRALITATEEDHARIAELLKVADVPKPTPVVRRVYALQN
LTFQEAQERLKPVLERELKGARLEGVPGNPKALLLEATEADQAFFAEILKAADVPPQVAPPTQEATVRRLYPLRFADAEK
VAPFLAREVPGIVVQTVPGQPVLSVRGTEKQLSEVETLLAQIDRAPEQGPPVFQRSYQLSNAKAADLAKVLQEALQARQA
QVPQSQAQPQAPTRQATVVADERTNTLIVTGTQEDLALVEGLIPKLDQAVPQVNLRVRIQEVQSNFSRNLGLKWNTIAGG
NVAASILDSGLSLIFDSTRSLAALNILATLEALQRQGLSRALRDVNQTVLNNQTARLQSGETFLIRRVVDDRVERVPFDI
GIIVEVTPQITADGQILLNIKAEVSGNVQRNPVDGDVDRFTKQVVTTTLRVRDGQTVVLGGLTSQENTQVQQGVPLLMDI
PLIGELFKQRSQETTDRELLVVITADILKETASR

Nucleotide


Download         Length: 2265 bp        

>NTDB_id=657533 MI302_RS09790 WP_239725643.1 1714563..1716827(+) (pilQ) [Thermus sp. NEB1569]
ATGAAACTTCTGATCCCCACCCTTTTGGCCCTGGGTGCCGGGGCATGGGCGGGAAGCCTTCCCCAGGAACCCCGCTTTGA
CGCCAAGGTGGACCTGAAGGTATCCGAAAGCCAGGTGCGGGCCGGGCTCACCCTGCCTCTGGATGTGGTCCTGGAAGCCC
TAGCCCGGAGCGTGGGCCTTCAGCCCCTTATCTACCGGGCCTACGATGCTTCCGGCGATCCAGCCAAGGCCCAGCCCCCC
TTACCCAACATCAAGTTGGACTTCCAGGGCAAACCCTTCCGGGAAGTCTGGGACCTCCTCTTCGCCACCTACGGCACCCA
GTTCAACCTGGACTACCTCTTCCTGCCTCCCGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCACCGCCTTGGTGGACG
CTCCAAGCCGCACCGGGGCCATGGAGCGGAAGCCCTACCTGGTGGCCATCCCCGAGATCGCCTATCGGCGCACGGAAACC
GACGCCCAGGGCCAGGCCCGCACCGTGGTGAACATAGATGGGGCCAAGGCCTGGGTGCAAAACGATCTCCTGCCCTTCCT
TTCCCGGGAAGCCAGCGGGCTTAGCGTGAACTGGATTGTGGTGGAGGAAGGGGGCAAACTCAGAGCCCTTCTCTCCGTTC
TAGCCACTCCCGAACAGCATACTCGCTTCTCGGACATCCTGCAGCGGGCAGGGATTGACTTCCGCCCCTTGCCCGCCCTC
ACCCTGCCCAAGCCTCGGGTAGAGCGCACCTACACCCTCACCCATGCCACCTTCCCCGAACTCCTCGCCTTCCTGCAAGG
CCAGGTGCCCAACGCCCAGATCGCCGTGGTCCCCACCGACCCCAAACGGGCCCTTATCACCGCCACCGAGGAGGACCACG
CCCGCATCGCCGAACTCCTGAAGGTGGCCGATGTACCCAAGCCCACTCCCGTGGTGCGCCGGGTCTACGCCTTGCAGAAC
CTCACCTTCCAGGAAGCCCAGGAAAGGCTTAAGCCCGTCCTGGAAAGGGAGTTGAAAGGGGCCCGCCTGGAAGGTGTCCC
GGGTAACCCCAAAGCCCTTCTCCTGGAGGCTACCGAAGCCGATCAGGCCTTCTTCGCCGAGATCCTCAAGGCCGCCGATG
TGCCTCCCCAGGTGGCCCCGCCCACCCAGGAGGCCACGGTGCGGAGGCTCTACCCCTTGCGCTTCGCCGATGCTGAAAAG
GTAGCTCCCTTCCTGGCTCGGGAGGTGCCGGGGATCGTGGTACAGACGGTGCCCGGGCAACCGGTCCTCTCGGTGCGGGG
CACGGAAAAGCAACTTTCCGAGGTGGAAACCCTCCTGGCCCAGATCGACCGGGCTCCGGAGCAAGGTCCCCCGGTGTTCC
AGCGTTCCTACCAGCTCTCCAACGCCAAGGCCGCCGACCTGGCCAAGGTGCTCCAGGAGGCCTTGCAAGCCCGGCAGGCC
CAGGTTCCCCAGAGCCAGGCCCAGCCCCAAGCCCCCACACGCCAGGCCACGGTGGTGGCGGACGAGCGCACCAATACCCT
GATCGTCACGGGCACACAGGAGGATCTGGCCCTGGTGGAAGGCCTTATTCCCAAGCTGGACCAGGCGGTGCCCCAGGTGA
ACCTGAGGGTGCGCATCCAAGAGGTGCAATCCAACTTCAGCCGAAACCTGGGTCTCAAGTGGAACACCATTGCCGGGGGA
AATGTGGCGGCCAGCATCCTGGACTCTGGGCTTTCCCTGATCTTTGACAGCACCCGGAGCCTGGCTGCCCTGAACATCCT
GGCCACCCTCGAGGCCCTCCAGCGCCAGGGTCTTTCCCGGGCCCTCAGGGATGTGAACCAGACCGTCCTCAACAACCAAA
CCGCCCGCCTGCAGTCCGGGGAGACCTTCCTCATCCGCCGGGTGGTGGATGACCGGGTGGAGCGGGTACCCTTTGACATC
GGCATCATCGTGGAGGTGACCCCCCAGATCACCGCCGACGGGCAGATCCTCCTCAACATCAAGGCGGAGGTTTCCGGAAA
CGTCCAGCGCAACCCGGTGGATGGGGATGTGGACCGCTTCACCAAGCAGGTGGTAACCACCACCTTAAGGGTGCGGGACG
GGCAGACCGTGGTCCTCGGGGGCCTCACCTCCCAGGAAAACACCCAGGTGCAACAGGGCGTGCCCCTTCTCATGGATATC
CCCCTGATTGGGGAACTCTTCAAGCAAAGGAGCCAGGAGACCACCGACCGGGAGCTACTGGTGGTCATCACCGCCGACAT
CTTGAAGGAAACCGCAAGCCGCTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(592-749)

Bacterial type II/III secretion system short domain (Secretin_N)

(455-532)

Bacterial type II/III secretion system short domain (Secretin_N)

(389-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Thermus thermophilus HB27

84.316

98.939

0.834