Detailed information NTG0657529

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MI302_RS09770
• Genome accession: NZ_CP092445
• Coordinates: 1711230..1712363 (+)
• Length: 377 a.a.
• NCBI ID: WP_239725640.1
• Uniprot ID: -
• Organism: Thermus sp. NEB1569
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1706230..1717363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MI302_RS09745 (MI302_09745)	-	1706964..1707950 (+)	987	WP_370687340.1	hypothetical protein	-
MI302_RS09750 (MI302_09750)	-	1707899..1708642 (+)	744	WP_135260897.1	transposase	-
MI302_RS09755 (MI302_09755)	-	1708676..1709428 (+)	753	Protein_1814	HD-GYP domain-containing protein	-
MI302_RS09760 (MI302_09760)	-	1709479..1709973 (+)	495	WP_239725639.1	DUF5317 domain-containing protein	-
MI302_RS09765 (MI302_09765)	-	1710046..1711050 (+)	1005	WP_054392201.1	homoisocitrate dehydrogenase	-
MI302_RS09770 (MI302_09770)	pilM	1711230..1712363 (+)	1134	WP_239725640.1	type IV pilus assembly protein PilM	Machinery gene
MI302_RS09775 (MI302_09775)	pilN	1712356..1712979 (+)	624	WP_239725641.1	flagellar protein FliT	Machinery gene
MI302_RS09780 (MI302_09780)	pilO	1712969..1713592 (+)	624	WP_015717936.1	type 4a pilus biogenesis protein PilO	Machinery gene
MI302_RS09785 (MI302_09785)	pilW	1713589..1714551 (+)	963	WP_239725642.1	competence protein	Machinery gene
MI302_RS09790 (MI302_09790)	pilQ	1714563..1716827 (+)	2265	WP_239725643.1	secretin N-terminal domain-containing protein	Machinery gene

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41307.88 Da
• Isoelectric Point: 4.4140

>NTDB_id=657529 MI302_RS09770 WP_239725640.1 1711230..1712363(+) (pilM) [Thermus sp. NEB1569]
MFSGFSKLFKPRVEALGLEIGTANLKLVELSGNPPALRALATRPTPPGMLVEGVIAEPQALAQELKELLAEARTKKRYVV
TAVPNPSVILRTLQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVADGEQVEVMVGAARQEAVASLLEAL
RGAGLTPIILDVKPFAGLYPLEAQLSSDPEGVSVAVEIGAESTSLVLLKGDRPLAVRILTLSGKDFTEAIGKSFGLDFLT
AEEVKRTYGLATIPTEDEELLLDFDAERERYSPAKIYDAIRPILVELTQEIRRSLEFFRVQLGDIQPEVGYLYGGGSRLR
GLPTLLTDTLGVNFTVPDPWQGIQVDPRRFDLEKLKELGPEFMVPVGLALRGVMPLD

Nucleotide

• Length: 1134 bp

>NTDB_id=657529 MI302_RS09770 WP_239725640.1 1711230..1712363(+) (pilM) [Thermus sp. NEB1569]
GTGTTTTCGGGTTTTAGCAAGCTATTCAAACCCCGCGTGGAGGCGCTGGGCCTGGAGATCGGCACCGCCAACCTAAAGCT
GGTGGAGCTCTCCGGAAATCCCCCCGCCCTCAGAGCCCTGGCCACCCGGCCCACTCCCCCAGGAATGCTGGTGGAAGGGG
TGATCGCCGAACCCCAGGCCCTGGCCCAGGAACTTAAGGAACTTCTCGCCGAAGCCCGGACCAAAAAGCGCTACGTGGTG
ACTGCCGTGCCCAATCCCAGCGTGATCCTGCGCACCCTTCAGGTGCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
TCGCTGGGAAGCGGAGCGGTACATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGGATCCCCTGGCCG
AGGTAGCCGATGGGGAGCAGGTGGAGGTCATGGTGGGGGCCGCCCGGCAGGAAGCAGTGGCCTCCTTGCTGGAAGCCCTG
CGGGGAGCAGGTCTGACCCCCATCATCTTGGATGTGAAGCCCTTTGCCGGGCTTTATCCCCTGGAAGCCCAGCTCAGCAG
CGACCCGGAAGGGGTTTCCGTGGCCGTAGAAATCGGGGCAGAAAGCACCAGCCTGGTTCTCCTTAAGGGAGACCGTCCCT
TGGCGGTGCGGATCCTCACCCTCTCCGGCAAGGACTTCACCGAAGCCATCGGGAAAAGCTTTGGCCTGGACTTCCTCACC
GCCGAGGAGGTGAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAGCTTCTTTTGGACTTTGACGCCGA
AAGGGAGCGGTACAGCCCCGCCAAAATCTACGATGCCATCCGGCCCATCCTGGTGGAACTCACTCAGGAGATCCGTCGGA
GCCTGGAGTTTTTCCGCGTGCAACTGGGTGACATACAGCCGGAGGTAGGGTACCTCTACGGCGGGGGAAGCCGGCTTAGG
GGCCTGCCCACCCTGCTTACCGATACCCTGGGGGTCAACTTCACCGTCCCCGACCCCTGGCAGGGCATCCAGGTGGATCC
CAGGCGCTTTGACCTGGAAAAGCTAAAGGAGCTGGGACCGGAGTTCATGGTGCCCGTGGGCCTGGCCTTACGGGGGGTGA
TGCCCCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-372)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	76.393	100	0.764