Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MI302_RS09780 Genome accession   NZ_CP092445
Coordinates   1712969..1713592 (+) Length   207 a.a.
NCBI ID   WP_015717936.1    Uniprot ID   A0A430UQ12
Organism   Thermus sp. NEB1569     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707969..1718592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS09755 (MI302_09755) - 1708676..1709428 (+) 753 Protein_1814 HD-GYP domain-containing protein -
  MI302_RS09760 (MI302_09760) - 1709479..1709973 (+) 495 WP_239725639.1 DUF5317 domain-containing protein -
  MI302_RS09765 (MI302_09765) - 1710046..1711050 (+) 1005 WP_054392201.1 homoisocitrate dehydrogenase -
  MI302_RS09770 (MI302_09770) pilM 1711230..1712363 (+) 1134 WP_239725640.1 type IV pilus assembly protein PilM Machinery gene
  MI302_RS09775 (MI302_09775) pilN 1712356..1712979 (+) 624 WP_239725641.1 flagellar protein FliT Machinery gene
  MI302_RS09780 (MI302_09780) pilO 1712969..1713592 (+) 624 WP_015717936.1 type 4a pilus biogenesis protein PilO Machinery gene
  MI302_RS09785 (MI302_09785) pilW 1713589..1714551 (+) 963 WP_239725642.1 competence protein Machinery gene
  MI302_RS09790 (MI302_09790) pilQ 1714563..1716827 (+) 2265 WP_239725643.1 secretin N-terminal domain-containing protein Machinery gene
  MI302_RS09795 (MI302_09795) aroC 1716931..1718082 (+) 1152 WP_019550802.1 chorismate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22808.19 Da        Isoelectric Point: 9.4989

>NTDB_id=657531 MI302_RS09780 WP_015717936.1 1712969..1713592(+) (pilO) [Thermus sp. NEB1569]
MLARLGQREWALIAIALTLVVALLWYFLLIVPMRQETESVRQEINALIPERDKGRQAQRALPELRATIAELQAERQAFLR
ALPKEERLSQVLNEILTEALRSGVTVRSFTRSPTSAPVPEVRAVNLALSLEAPFPETYAYLQRLEGLSRFSSISGLNLSV
QGQEVNPSLNTSLTLTLYVLAKDLGQSEGTTKTQATPSPQTGQGGGR

Nucleotide


Download         Length: 624 bp        

>NTDB_id=657531 MI302_RS09780 WP_015717936.1 1712969..1713592(+) (pilO) [Thermus sp. NEB1569]
GTGCTCGCTAGACTTGGACAGCGGGAATGGGCCCTGATCGCCATCGCCCTCACCTTGGTGGTGGCCCTCCTCTGGTACTT
CCTCCTCATCGTTCCCATGCGCCAGGAAACGGAAAGCGTGCGCCAGGAGATCAACGCCCTTATTCCCGAACGGGATAAAG
GAAGGCAAGCCCAGCGCGCCCTTCCAGAACTCCGGGCCACCATCGCTGAGCTACAAGCCGAGCGCCAAGCCTTTCTGAGG
GCGCTTCCCAAGGAGGAACGGCTTTCCCAGGTCCTGAACGAGATCCTGACCGAAGCCCTAAGGAGCGGGGTCACGGTGCG
TTCCTTCACCCGCTCCCCCACCTCGGCCCCGGTGCCGGAGGTGCGGGCGGTGAACCTGGCCCTTTCCCTCGAGGCCCCCT
TCCCCGAAACCTACGCCTACCTACAGCGTCTGGAAGGGCTTTCCCGCTTCAGCTCCATCTCCGGGCTGAACCTGAGCGTC
CAGGGTCAGGAGGTTAACCCAAGCCTCAACACCAGCCTGACCCTCACCCTCTACGTTCTGGCCAAAGACCTAGGCCAGTC
TGAAGGAACCACAAAGACGCAAGCAACCCCATCGCCGCAAACAGGCCAAGGAGGTGGTCGGTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A430UQ12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

73.016

91.304

0.667