Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   MI302_RS09785 Genome accession   NZ_CP092445
Coordinates   1713589..1714551 (+) Length   320 a.a.
NCBI ID   WP_239725642.1    Uniprot ID   -
Organism   Thermus sp. NEB1569     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1708589..1719551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS09755 (MI302_09755) - 1708676..1709428 (+) 753 Protein_1814 HD-GYP domain-containing protein -
  MI302_RS09760 (MI302_09760) - 1709479..1709973 (+) 495 WP_239725639.1 DUF5317 domain-containing protein -
  MI302_RS09765 (MI302_09765) - 1710046..1711050 (+) 1005 WP_054392201.1 homoisocitrate dehydrogenase -
  MI302_RS09770 (MI302_09770) pilM 1711230..1712363 (+) 1134 WP_239725640.1 type IV pilus assembly protein PilM Machinery gene
  MI302_RS09775 (MI302_09775) pilN 1712356..1712979 (+) 624 WP_239725641.1 flagellar protein FliT Machinery gene
  MI302_RS09780 (MI302_09780) pilO 1712969..1713592 (+) 624 WP_015717936.1 type 4a pilus biogenesis protein PilO Machinery gene
  MI302_RS09785 (MI302_09785) pilW 1713589..1714551 (+) 963 WP_239725642.1 competence protein Machinery gene
  MI302_RS09790 (MI302_09790) pilQ 1714563..1716827 (+) 2265 WP_239725643.1 secretin N-terminal domain-containing protein Machinery gene
  MI302_RS09795 (MI302_09795) aroC 1716931..1718082 (+) 1152 WP_019550802.1 chorismate synthase -
  MI302_RS09800 (MI302_09800) - 1718100..1718654 (+) 555 WP_015717940.1 shikimate kinase -

Sequence

Protein


Download         Length: 320 a.a.        Molecular weight: 32980.24 Da        Isoelectric Point: 5.4487

>NTDB_id=657532 MI302_RS09785 WP_239725642.1 1713589..1714551(+) (pilW) [Thermus sp. NEB1569]
MKALLARLATAWRNLPQSTKLLLAGLLLVSAVALWYVGFYLPAQAPMEVQPTPGQPQVPSQGAEAPKAIEAPPIPPLAET
APQDQAAKPSPEASPSQKAPKAGEPVLTTALPIPKTQQEAPLPNPFVPLVVEAPPSPPVPSPAPAPRPTPVPTGAPVRVT
QGTPLPTPSVQASPRPLPGSQGALPAPKVLTPAFQVETPKAQVDTPPPLTPPAGLVEAPLPRAPQTPEGGKTESSPTTSP
KTPLQTLVEERGLKLTGTLLGPVSVAILESKEGYLVLPVGSLLPGSEAVLRRIESDRVVLALKDESLEIVLDSMQAGGGQ

Nucleotide


Download         Length: 963 bp        

>NTDB_id=657532 MI302_RS09785 WP_239725642.1 1713589..1714551(+) (pilW) [Thermus sp. NEB1569]
GTGAAAGCCCTTCTAGCGCGCCTGGCCACGGCTTGGCGCAACCTACCCCAGTCCACCAAGCTCCTCCTGGCGGGCCTTCT
CCTGGTGAGCGCCGTGGCGCTTTGGTATGTGGGTTTCTACCTGCCCGCCCAGGCACCCATGGAGGTGCAACCCACCCCCG
GCCAACCTCAGGTGCCCAGCCAGGGAGCGGAGGCTCCCAAGGCCATAGAAGCTCCACCCATCCCGCCCCTTGCCGAAACT
GCCCCCCAAGACCAAGCGGCGAAGCCTAGCCCCGAGGCTTCTCCTTCCCAGAAAGCCCCTAAAGCAGGCGAGCCCGTCTT
GACCACAGCCTTGCCCATCCCCAAAACGCAACAAGAGGCCCCGCTTCCAAACCCCTTTGTGCCCCTGGTGGTGGAAGCTC
CACCTTCTCCCCCCGTGCCCTCCCCTGCCCCGGCACCTAGGCCCACCCCCGTACCCACAGGGGCCCCTGTCCGGGTAACC
CAGGGAACACCCTTGCCCACCCCAAGCGTTCAAGCTTCACCCCGGCCTCTCCCGGGAAGCCAGGGAGCCCTGCCCGCTCC
CAAGGTGCTTACCCCTGCTTTCCAGGTGGAAACCCCCAAAGCCCAGGTGGACACTCCACCTCCCCTCACCCCACCCGCTG
GCCTTGTGGAGGCTCCTTTACCCAGGGCACCCCAAACTCCAGAGGGAGGAAAAACTGAATCTTCCCCCACCACTTCCCCC
AAAACCCCCTTGCAGACCCTGGTGGAGGAAAGGGGTCTCAAGCTAACCGGCACCCTTCTGGGTCCGGTGAGCGTGGCTAT
CCTGGAAAGCAAGGAGGGTTACTTGGTGCTACCCGTGGGTAGCCTTCTTCCAGGTAGCGAGGCAGTGCTTCGCCGCATAG
AAAGCGACCGGGTGGTGTTGGCCTTGAAGGATGAAAGCCTTGAGATTGTCCTAGACAGCATGCAAGCAGGAGGTGGCCAG
TGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

52.632

100

0.531