Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   MI302_RS04610 Genome accession   NZ_CP092445
Coordinates   740748..741218 (-) Length   156 a.a.
NCBI ID   WP_239726354.1    Uniprot ID   -
Organism   Thermus sp. NEB1569     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735748..746218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS04580 (MI302_04580) comZ 736145..736330 (-) 186 WP_239726349.1 hypothetical protein Machinery gene
  MI302_RS04585 (MI302_04585) - 736332..737075 (-) 744 WP_135260897.1 transposase -
  MI302_RS04590 (MI302_04590) comZ 737024..738907 (-) 1884 WP_239726350.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  MI302_RS04595 (MI302_04595) pilA/pilA3 738918..739619 (-) 702 WP_239726351.1 PilW family protein Machinery gene
  MI302_RS04600 (MI302_04600) pilA/pilA2 739637..740224 (-) 588 WP_239726352.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MI302_RS04605 (MI302_04605) - 740221..740694 (-) 474 WP_239726353.1 GspH/FimT family protein -
  MI302_RS04610 (MI302_04610) pilA/pilA1 740748..741218 (-) 471 WP_239726354.1 GspH/FimT family protein Machinery gene
  MI302_RS04615 (MI302_04615) - 741285..742475 (+) 1191 WP_239726355.1 thiolase family protein -
  MI302_RS04620 (MI302_04620) - 742477..742785 (-) 309 WP_019551627.1 DUF503 domain-containing protein -
  MI302_RS04625 (MI302_04625) - 742842..743201 (-) 360 WP_038030453.1 DUF86 domain-containing protein -
  MI302_RS04630 (MI302_04630) - 743179..743490 (-) 312 WP_028493102.1 nucleotidyltransferase family protein -
  MI302_RS04635 (MI302_04635) - 743500..745965 (-) 2466 WP_239726356.1 outer membrane protein assembly factor -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16700.38 Da        Isoelectric Point: 10.5430

>NTDB_id=657523 MI302_RS04610 WP_239726354.1 740748..741218(-) (pilA/pilA1) [Thermus sp. NEB1569]
MAQRGVTLLELLLVLGVMGVLLALGLPLLSPQQLALDQAARSLAAQVTRARLEAIRRNAPAGLHLFTEGAGGYAVFVDRD
GDWGYDPGEEVQLVRFGQGEWARVRLDLGQSTLGNLPLLFDPRGVPARPITAALVLRAGTATRKVVVSQQGRARVE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=657523 MI302_RS04610 WP_239726354.1 740748..741218(-) (pilA/pilA1) [Thermus sp. NEB1569]
ATGGCCCAGCGGGGCGTTACCCTTCTGGAACTCCTCCTGGTCTTGGGCGTTATGGGGGTTCTCCTCGCCCTCGGCCTTCC
CCTTCTCTCCCCTCAGCAGCTGGCCCTGGATCAGGCGGCCCGTTCCCTGGCGGCCCAGGTAACCCGGGCCAGGCTCGAGG
CCATCCGCCGAAACGCTCCTGCTGGGCTTCACCTCTTCACCGAGGGGGCGGGGGGCTACGCCGTCTTCGTGGACCGGGAT
GGGGACTGGGGCTATGACCCGGGGGAAGAGGTGCAGCTTGTTCGCTTTGGCCAGGGGGAATGGGCCCGGGTACGCCTGGA
CCTGGGGCAGAGCACCCTGGGCAACCTCCCCCTCCTCTTTGACCCCCGGGGCGTGCCCGCCCGGCCCATTACCGCTGCCC
TCGTCCTAAGGGCGGGCACGGCCACCCGGAAGGTGGTGGTGAGCCAGCAGGGCCGGGCCCGGGTGGAATGA

Domains

Predicted by InterproScan.

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

73.228

81.41

0.596