NTDB

Overview

Name	pilA/pilA3	Type	Machinery gene
Locus tag	MI302_RS04595	Genome accession	NZ_CP092445
Coordinates	738918..739619 (-)	Length	233 a.a.
NCBI ID	WP_239726351.1	Uniprot ID	-
Organism	Thermus sp. NEB1569
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 733918..744619

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MI302_RS04560 (MI302_04560)	-	733969..734229 (-)	261	WP_239726348.1	hypothetical protein	-
MI302_RS04565 (MI302_04565)	-	734290..734796 (-)	507	WP_008633626.1	IS630 family transposase	-
MI302_RS04570 (MI302_04570)	-	734835..735362 (-)	528	WP_019551665.1	winged helix-turn-helix domain-containing protein	-
MI302_RS04575 (MI302_04575)	-	735573..735932 (-)	360	WP_126204502.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
MI302_RS04580 (MI302_04580)	comZ	736145..736330 (-)	186	WP_239726349.1	hypothetical protein	Machinery gene
MI302_RS04585 (MI302_04585)	-	736332..737075 (-)	744	WP_135260897.1	transposase	-
MI302_RS04590 (MI302_04590)	comZ	737024..738907 (-)	1884	WP_239726350.1	pilus assembly PilX N-terminal domain-containing protein	Machinery gene
MI302_RS04595 (MI302_04595)	pilA/pilA3	738918..739619 (-)	702	WP_239726351.1	PilW family protein	Machinery gene
MI302_RS04600 (MI302_04600)	pilA/pilA2	739637..740224 (-)	588	WP_239726352.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
MI302_RS04605 (MI302_04605)	-	740221..740694 (-)	474	WP_239726353.1	GspH/FimT family protein	-
MI302_RS04610 (MI302_04610)	pilA/pilA1	740748..741218 (-)	471	WP_239726354.1	GspH/FimT family protein	Machinery gene
MI302_RS04615 (MI302_04615)	-	741285..742475 (+)	1191	WP_239726355.1	thiolase family protein	-
MI302_RS04620 (MI302_04620)	-	742477..742785 (-)	309	WP_019551627.1	DUF503 domain-containing protein	-
MI302_RS04625 (MI302_04625)	-	742842..743201 (-)	360	WP_038030453.1	DUF86 domain-containing protein	-
MI302_RS04630 (MI302_04630)	-	743179..743490 (-)	312	WP_028493102.1	nucleotidyltransferase family protein	-

Sequence

Protein

Download Length: 233 a.a. Molecular weight: 24953.37 Da Isoelectric Point: 4.9648

                                >NTDB_id=657521 MI302_RS04595 WP_239726351.1 738918..739619(-) (pilA/pilA3) [Thermus sp. NEB1569]

MRKGFTLVEVLVAMAILVVVLALGVRYFAATGELARNTQGRNELQDRVRMVMQVVTGDLQMAGARYWNSGSQNRAYGLPL

PPLEGEDGGAKDTLTLYYVTSLRDPASACRRVDYGFAGDTLLRSDLNATPTSGSDCTSPTPNFQPLAEGILALDIQYQCS

DGTTQDQPECNPDAYPRSAKVTVAGYSLSPVTNPGPASLTTVTHQTLPCPPGRACYALTQEVLMPNLKPLPAP

Nucleotide

Download Length: 702 bp

                                >NTDB_id=657521 MI302_RS04595 WP_239726351.1 738918..739619(-) (pilA/pilA3) [Thermus sp. NEB1569]

ATGAGGAAGGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATTTTGGTGGTGGTCCTGGCCCTTGGGGTGCGCTA

CTTCGCCGCCACGGGCGAGCTCGCTCGGAACACCCAGGGGCGGAACGAGCTTCAGGACCGGGTGCGGATGGTCATGCAGG

TGGTGACCGGCGATCTGCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAGCCAAAACCGGGCCTACGGCCTGCCCCTT

CCCCCCCTTGAGGGGGAGGATGGGGGTGCCAAGGACACCCTGACCCTCTACTACGTCACTAGCCTTAGGGACCCTGCCTC

CGCCTGCCGGCGGGTGGACTATGGGTTTGCCGGGGATACGCTCCTAAGGAGCGACCTGAACGCCACGCCCACCTCCGGAA

GCGACTGCACCTCCCCTACCCCCAACTTCCAGCCCCTGGCCGAGGGCATCCTCGCCCTGGACATCCAGTACCAGTGCAGC

GACGGTACCACCCAAGACCAGCCGGAGTGCAACCCAGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC

CCTAAGCCCCGTGACCAACCCGGGGCCCGCCTCCCTCACCACCGTCACCCACCAAACCCTCCCTTGCCCCCCGGGCCGGG

CCTGCTACGCCCTCACCCAGGAGGTTCTCATGCCCAACCTCAAGCCCTTGCCCGCCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilA3	Thermus thermophilus HB27	82.833	100	0.828

Detailed information NTG0657521