Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   MIS33_RS08540 Genome accession   NZ_CP092359
Coordinates   1715552..1715884 (+) Length   110 a.a.
NCBI ID   WP_249451468.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1684742..1748582 1715552..1715884 within 0


Gene organization within MGE regions


Location: 1684742..1748582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS08360 (MIS33_08360) - 1684742..1685185 (-) 444 WP_249451442.1 hypothetical protein -
  MIS33_RS08365 (MIS33_08365) - 1685182..1685505 (-) 324 WP_249447501.1 hypothetical protein -
  MIS33_RS08370 (MIS33_08370) - 1685502..1685984 (-) 483 WP_249451443.1 M15 family metallopeptidase -
  MIS33_RS08375 (MIS33_08375) - 1686021..1686350 (-) 330 WP_249451444.1 hypothetical protein -
  MIS33_RS08380 (MIS33_08380) - 1686323..1686772 (-) 450 WP_249447498.1 hypothetical protein -
  MIS33_RS08385 (MIS33_08385) - 1686928..1687230 (-) 303 WP_249451445.1 hypothetical protein -
  MIS33_RS08390 (MIS33_08390) - 1687288..1692354 (-) 5067 WP_249451446.1 phage tail protein -
  MIS33_RS08395 (MIS33_08395) - 1692360..1693076 (-) 717 WP_249451447.1 tail assembly protein -
  MIS33_RS08400 (MIS33_08400) - 1693165..1693443 (+) 279 WP_249447494.1 type II toxin-antitoxin system RelE/ParE family toxin -
  MIS33_RS08405 (MIS33_08405) - 1693501..1693809 (+) 309 WP_249447493.1 HigA family addiction module antitoxin -
  MIS33_RS08410 (MIS33_08410) - 1693845..1694600 (-) 756 WP_249451448.1 C40 family peptidase -
  MIS33_RS08415 (MIS33_08415) - 1694579..1695286 (-) 708 WP_249451604.1 phage minor tail protein L -
  MIS33_RS08420 (MIS33_08420) - 1695298..1695627 (-) 330 WP_249451449.1 phage tail protein -
  MIS33_RS08425 (MIS33_08425) - 1695698..1696162 (-) 465 WP_249451450.1 hypothetical protein -
  MIS33_RS08430 (MIS33_08430) - 1696256..1699582 (-) 3327 WP_249451451.1 phage tail tape measure protein -
  MIS33_RS08435 (MIS33_08435) - 1699579..1699875 (-) 297 WP_249451452.1 DUF1799 domain-containing protein -
  MIS33_RS08440 (MIS33_08440) - 1699893..1700207 (-) 315 WP_249447488.1 phage tail assembly chaperone -
  MIS33_RS08445 (MIS33_08445) - 1700257..1700895 (-) 639 WP_249451453.1 phage tail protein -
  MIS33_RS08450 (MIS33_08450) - 1700961..1701122 (-) 162 WP_249447486.1 hypothetical protein -
  MIS33_RS08455 (MIS33_08455) - 1701119..1701469 (-) 351 WP_249451454.1 DUF3168 domain-containing protein -
  MIS33_RS08460 (MIS33_08460) - 1701462..1701932 (-) 471 WP_249451455.1 HK97-gp10 family putative phage morphogenesis protein -
  MIS33_RS08465 (MIS33_08465) - 1701929..1702174 (-) 246 WP_249451456.1 hypothetical protein -
  MIS33_RS08470 (MIS33_08470) - 1702181..1702498 (-) 318 WP_249451457.1 phage head closure protein -
  MIS33_RS08475 (MIS33_08475) - 1702508..1703212 (-) 705 WP_249451458.1 3'-5' exonuclease -
  MIS33_RS08480 (MIS33_08480) - 1703295..1703591 (-) 297 WP_249451459.1 head-tail connector protein -
  MIS33_RS08485 (MIS33_08485) - 1703591..1703902 (-) 312 WP_249451460.1 hypothetical protein -
  MIS33_RS08490 (MIS33_08490) - 1703996..1705228 (-) 1233 WP_249451461.1 phage major capsid protein -
  MIS33_RS08495 (MIS33_08495) - 1705230..1706060 (-) 831 WP_249451462.1 head maturation protease, ClpP-related -
  MIS33_RS08500 (MIS33_08500) - 1706057..1707289 (-) 1233 WP_249451463.1 phage portal protein -
  MIS33_RS08505 (MIS33_08505) - 1707346..1709040 (-) 1695 WP_249451464.1 terminase large subunit -
  MIS33_RS08510 (MIS33_08510) - 1709042..1709629 (-) 588 WP_249447474.1 hypothetical protein -
  MIS33_RS11725 - 1709791..1710102 (-) 312 WP_283397656.1 HNH endonuclease signature motif containing protein -
  MIS33_RS08520 (MIS33_08520) - 1710236..1710628 (-) 393 WP_249451465.1 hypothetical protein -
  MIS33_RS08525 (MIS33_08525) - 1711152..1714040 (-) 2889 WP_249451466.1 DUF5906 domain-containing protein -
  MIS33_RS08530 (MIS33_08530) - 1714203..1714397 (-) 195 WP_249447471.1 Cro/CI family transcriptional regulator -
  MIS33_RS08535 (MIS33_08535) - 1714519..1715091 (+) 573 WP_249451467.1 helix-turn-helix domain-containing protein -
  MIS33_RS08540 (MIS33_08540) ssb 1715552..1715884 (+) 333 WP_249451468.1 single-stranded DNA-binding protein Machinery gene
  MIS33_RS08545 (MIS33_08545) - 1715887..1716207 (+) 321 WP_249451469.1 hypothetical protein -
  MIS33_RS08550 (MIS33_08550) - 1716217..1716435 (+) 219 WP_249451470.1 hypothetical protein -
  MIS33_RS08555 (MIS33_08555) - 1716507..1717223 (+) 717 WP_249451471.1 hypothetical protein -
  MIS33_RS08560 (MIS33_08560) - 1717186..1717440 (+) 255 WP_249451472.1 flagellar protein required for flagellar formation -
  MIS33_RS08565 (MIS33_08565) - 1717467..1718348 (+) 882 WP_249451473.1 hypothetical protein -
  MIS33_RS08570 (MIS33_08570) - 1718360..1718557 (+) 198 WP_249451474.1 hypothetical protein -
  MIS33_RS08575 (MIS33_08575) - 1718597..1719805 (-) 1209 WP_249451475.1 integrase arm-type DNA-binding domain-containing protein -
  MIS33_RS08585 (MIS33_08585) - 1720159..1720644 (-) 486 WP_249442133.1 GNAT family N-acetyltransferase -
  MIS33_RS08590 (MIS33_08590) - 1720842..1721633 (+) 792 WP_249442134.1 basic amino acid ABC transporter substrate-binding protein -
  MIS33_RS08595 (MIS33_08595) - 1721733..1722323 (+) 591 WP_249447459.1 hypothetical protein -
  MIS33_RS08600 (MIS33_08600) alaS 1722598..1725489 (+) 2892 WP_249447458.1 alanine--tRNA ligase -
  MIS33_RS08605 (MIS33_08605) - 1725578..1727563 (+) 1986 WP_249447457.1 N-6 DNA methylase -
  MIS33_RS08610 (MIS33_08610) - 1727611..1728990 (+) 1380 WP_249451476.1 restriction endonuclease subunit S -
  MIS33_RS08615 (MIS33_08615) - 1729032..1729748 (+) 717 WP_249447455.1 restriction endonuclease subunit S -
  MIS33_RS08620 (MIS33_08620) - 1729745..1730572 (-) 828 WP_249447454.1 DUF72 domain-containing protein -
  MIS33_RS08625 (MIS33_08625) - 1730820..1731353 (+) 534 WP_249447453.1 NUDIX hydrolase -
  MIS33_RS08630 (MIS33_08630) gloB 1731350..1732114 (+) 765 WP_249447452.1 hydroxyacylglutathione hydrolase -
  MIS33_RS08640 (MIS33_08640) - 1732365..1732757 (-) 393 WP_249447695.1 diacylglycerol kinase -
  MIS33_RS08645 (MIS33_08645) gmhB 1732919..1733488 (+) 570 WP_249447451.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  MIS33_RS08650 (MIS33_08650) - 1733485..1734450 (+) 966 WP_249447450.1 glycosyltransferase family 2 protein -
  MIS33_RS08655 (MIS33_08655) menA 1734335..1735414 (+) 1080 WP_249447449.1 1,4-dihydroxy-2-naphthoate octaprenyltransferase -
  MIS33_RS08660 (MIS33_08660) - 1735565..1736032 (+) 468 WP_249447448.1 DUF4149 domain-containing protein -
  MIS33_RS08665 (MIS33_08665) - 1736293..1736772 (+) 480 WP_249447447.1 hypothetical protein -
  MIS33_RS08670 (MIS33_08670) - 1736776..1737565 (+) 790 Protein_1681 glycosyltransferase -
  MIS33_RS08675 (MIS33_08675) - 1737642..1738874 (+) 1233 WP_249451477.1 exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase -
  MIS33_RS08680 (MIS33_08680) yaaA 1739042..1739815 (+) 774 WP_249451478.1 peroxide stress protein YaaA -
  MIS33_RS08685 (MIS33_08685) kdsA 1739997..1740842 (-) 846 WP_249447442.1 3-deoxy-8-phosphooctulonate synthase -
  MIS33_RS08690 (MIS33_08690) - 1740978..1741946 (-) 969 WP_283397673.1 KpsF/GutQ family sugar-phosphate isomerase -
  MIS33_RS08695 (MIS33_08695) - 1742235..1742774 (+) 540 WP_249447694.1 HAD-IIIA family hydrolase -
  MIS33_RS08700 (MIS33_08700) lptC 1742771..1743349 (+) 579 WP_249447440.1 LPS export ABC transporter periplasmic protein LptC -
  MIS33_RS08705 (MIS33_08705) lptA 1743327..1743848 (+) 522 WP_249442155.1 lipopolysaccharide transport periplasmic protein LptA -
  MIS33_RS08710 (MIS33_08710) - 1743861..1744499 (+) 639 WP_249447439.1 FMN-binding negative transcriptional regulator -
  MIS33_RS08715 (MIS33_08715) lptB 1744552..1745280 (+) 729 WP_249442157.1 LPS export ABC transporter ATP-binding protein -
  MIS33_RS08720 (MIS33_08720) rpoN 1745345..1746703 (+) 1359 WP_249447438.1 RNA polymerase factor sigma-54 -
  MIS33_RS08725 (MIS33_08725) raiA 1746762..1747046 (+) 285 WP_249442159.1 ribosome-associated translation inhibitor RaiA -
  MIS33_RS08730 (MIS33_08730) - 1747015..1747188 (-) 174 WP_249447437.1 hypothetical protein -
  MIS33_RS08735 (MIS33_08735) rsmG 1747180..1747821 (+) 642 WP_249442160.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  MIS33_RS08740 (MIS33_08740) - 1747818..1748582 (+) 765 WP_249445572.1 AAA family ATPase -

Sequence


Protein


Download         Length: 110 a.a.        Molecular weight: 12757.62 Da        Isoelectric Point: 7.3831

>NTDB_id=656727 MIS33_RS08540 WP_249451468.1 1715552..1715884(+) (ssb) [Wielerella bovis strain 08LB461]
MHNLVILIGNLGKDPEIRHMPDGDPVASFSLATSETWIDKQGQKRETTQWHNIVCYRGTAKIAQDWLKKGKLICIEGKIV
YRQWQDEHQVTHYKTEIIAEQIQMLGKKDD

Nucleotide


Download         Length: 333 bp        

>NTDB_id=656727 MIS33_RS08540 WP_249451468.1 1715552..1715884(+) (ssb) [Wielerella bovis strain 08LB461]
ATGCACAATCTCGTTATTTTAATCGGCAATTTGGGCAAAGACCCCGAAATCCGCCATATGCCCGATGGCGACCCCGTTGC
CAGTTTTTCTCTTGCCACATCCGAAACTTGGATAGACAAGCAAGGGCAGAAACGCGAAACAACCCAATGGCACAACATCG
TTTGCTATCGTGGCACAGCCAAAATCGCACAAGATTGGCTCAAAAAAGGCAAACTCATTTGCATTGAAGGAAAAATTGTT
TACCGCCAATGGCAAGATGAGCATCAAGTAACTCACTACAAAACAGAAATTATTGCCGAACAAATTCAAATGCTTGGCAA
AAAGGATGATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Neisseria meningitidis MC58

49.074

98.182

0.482

  ssb Neisseria gonorrhoeae MS11

49.074

98.182

0.482

  ssb Glaesserella parasuis strain SC1401

50

94.545

0.473

  ssb Vibrio cholerae strain A1552

45.946

100

0.464