Detailed information NTG0656529

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MIH17_RS03070
• Genome accession: NZ_CP092288
• Coordinates: 646490..647611 (+)
• Length: 373 a.a.
• NCBI ID: WP_249007510.1
• Uniprot ID: -
• Organism: Marinobacter sp. M2C
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 641490..652611

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MIH17_RS03050 (MIH17_03050)	-	643027..643611 (-)	585	WP_249007506.1	YggT family protein	-
MIH17_RS03055 (MIH17_03055)	proC	643637..644467 (-)	831	WP_249007507.1	pyrroline-5-carboxylate reductase	-
MIH17_RS03060 (MIH17_03060)	-	644541..645245 (-)	705	WP_249007508.1	YggS family pyridoxal phosphate-dependent enzyme	-
MIH17_RS03065 (MIH17_03065)	pilT	645382..646416 (+)	1035	WP_249007509.1	type IV pilus twitching motility protein PilT	Machinery gene
MIH17_RS03070 (MIH17_03070)	pilU	646490..647611 (+)	1122	WP_249007510.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MIH17_RS03075 (MIH17_03075)	-	647729..649159 (+)	1431	WP_249007511.1	outer membrane protein transport protein	-
MIH17_RS03080 (MIH17_03080)	polA	649256..652051 (-)	2796	WP_249007512.1	DNA polymerase I	-
MIH17_RS03085 (MIH17_03085)	-	652044..652217 (-)	174	WP_249007513.1	hypothetical protein	-
MIH17_RS03090 (MIH17_03090)	-	652247..652582 (+)	336	WP_249007514.1	DUF2782 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41438.74 Da
• Isoelectric Point: 6.6614

>NTDB_id=656529 MIH17_RS03070 WP_249007510.1 646490..647611(+) (pilU) [Marinobacter sp. M2C]
MEFEKLLRLMVEKGGSDLFITAGVAPSMKVNGKVWPVTKTALTPDMTREMVYGAMSDKQRAEFDESHECNFAISARGIGR
FRVSAFFQRNQCGMVLRRIEVKIPEIDDLSLPDIVKELAMTKRGLIMFVGATGTGKSTSLAAMIGFRNHNSRGHIISIED
PIEFIHQHQGCIVTQREVGIDTASFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHGQIWMDLSLNLRAIVAQQLVPTPDGKGRKAVIEVLINTPLAADLIRKGEVHKLKDLMAKSNEIGMRTFDQA
LYELYSEGSVTYEDALAHADSPNDLRLMIKLGTDAKGVDKLSSSVDKLTIQQD

Nucleotide

• Length: 1122 bp

>NTDB_id=656529 MIH17_RS03070 WP_249007510.1 646490..647611(+) (pilU) [Marinobacter sp. M2C]
ATGGAATTTGAAAAGCTACTGCGGCTGATGGTTGAGAAAGGCGGCTCGGACTTGTTCATTACCGCCGGCGTTGCGCCCAG
TATGAAAGTGAACGGTAAAGTGTGGCCTGTCACAAAGACCGCCCTAACACCCGATATGACCCGTGAGATGGTTTACGGTG
CCATGAGCGACAAGCAACGCGCCGAGTTTGATGAAAGCCACGAGTGTAACTTCGCCATCAGCGCCCGTGGCATTGGTCGC
TTTCGGGTTAGCGCGTTTTTTCAGCGCAATCAGTGCGGTATGGTGCTGCGTCGTATTGAAGTAAAGATACCGGAAATCGA
CGATCTTTCACTGCCCGACATCGTTAAAGAGCTGGCTATGACCAAGCGCGGGCTGATCATGTTTGTCGGCGCCACCGGCA
CGGGCAAATCCACCTCGTTGGCTGCGATGATTGGCTTCCGCAACCACAACAGCCGCGGCCATATCATTTCTATTGAAGAC
CCGATTGAGTTTATACACCAACATCAGGGTTGCATTGTGACCCAGCGCGAAGTGGGCATAGATACTGCCAGCTTTGAAGT
GGCGTTGAAAAACACGCTGCGCCAGGCGCCAGATGTGATTTTGATTGGTGAGGTGCGAACCCGCCAGACCATGGAATATT
CAGTACAGTTCGCCGAAACCGGGCACTTGTGCCTGGCGACTCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTTTTTCCACCCGAACAGCACGGCCAAATCTGGATGGACCTGTCGCTGAACCTTCGCGCTATTGTTGCGCAGCA
ACTGGTGCCCACGCCAGACGGCAAGGGCCGGAAAGCGGTTATTGAAGTGCTGATCAATACGCCGCTGGCGGCTGACCTGA
TTCGCAAAGGCGAAGTGCACAAGCTCAAAGACCTGATGGCCAAATCCAACGAAATTGGTATGCGCACTTTTGATCAGGCG
CTGTATGAGCTGTATTCCGAAGGTTCCGTGACTTATGAAGATGCCTTGGCACATGCTGACTCACCCAACGATTTGCGTTT
GATGATAAAGCTTGGAACCGACGCCAAAGGGGTGGACAAGTTGTCGTCTTCGGTGGATAAGCTGACCATTCAGCAGGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	78.706	99.464	0.783
pilU	Acinetobacter baylyi ADP1	65.449	95.442	0.625
pilU	Vibrio cholerae strain A1552	54.324	99.196	0.539
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.563	95.174	0.386