Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LGL65_RS18355 Genome accession   NZ_CP092110
Coordinates   3562212..3562826 (+) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain LOH112     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3557212..3567826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGL65_RS18320 aroE 3557899..3558741 (+) 843 WP_017418183.1 shikimate dehydrogenase -
  LGL65_RS18325 yhbY 3558735..3559025 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  LGL65_RS18330 - 3559036..3559605 (+) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  LGL65_RS18335 yqeK 3559595..3560176 (+) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LGL65_RS18340 rsfS 3560173..3560529 (+) 357 WP_003152864.1 ribosome silencing factor -
  LGL65_RS18345 - 3560526..3561263 (+) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  LGL65_RS18350 comER 3561332..3562153 (-) 822 WP_014305449.1 late competence protein ComER -
  LGL65_RS18355 comEA 3562212..3562826 (+) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  LGL65_RS18360 - 3562893..3563462 (+) 570 WP_003152868.1 ComE operon protein 2 -
  LGL65_RS18365 comEC 3563463..3565814 (+) 2352 WP_025649830.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LGL65_RS18370 - 3565833..3565967 (-) 135 WP_003152870.1 YqzM family protein -
  LGL65_RS18375 - 3566008..3566159 (+) 152 Protein_3571 hypothetical protein -
  LGL65_RS18380 holA 3566199..3567294 (+) 1096 Protein_3572 DNA polymerase III subunit delta -
  LGL65_RS18385 rpsT 3567257..3567523 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=655986 LGL65_RS18355 WP_013352942.1 3562212..3562826(+) (comEA) [Bacillus velezensis strain LOH112]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=655986 LGL65_RS18355 WP_013352942.1 3562212..3562826(+) (comEA) [Bacillus velezensis strain LOH112]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363