Detailed information NTG0655988

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: LGL65_RS18365
• Genome accession: NZ_CP092110
• Coordinates: 3563463..3565814 (+)
• Length: 783 a.a.
• NCBI ID: WP_025649830.1
• Uniprot ID: A0A411A869
• Organism: Bacillus velezensis strain LOH112
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3558463..3570814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGL65_RS18325	yhbY	3558735..3559025 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LGL65_RS18330	-	3559036..3559605 (+)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
LGL65_RS18335	yqeK	3559595..3560176 (+)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LGL65_RS18340	rsfS	3560173..3560529 (+)	357	WP_003152864.1	ribosome silencing factor	-
LGL65_RS18345	-	3560526..3561263 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
LGL65_RS18350	comER	3561332..3562153 (-)	822	WP_014305449.1	late competence protein ComER	-
LGL65_RS18355	comEA	3562212..3562826 (+)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
LGL65_RS18360	-	3562893..3563462 (+)	570	WP_003152868.1	ComE operon protein 2	-
LGL65_RS18365	comEC	3563463..3565814 (+)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LGL65_RS18370	-	3565833..3565967 (-)	135	WP_003152870.1	YqzM family protein	-
LGL65_RS18375	-	3566008..3566159 (+)	152	Protein_3571	hypothetical protein	-
LGL65_RS18380	holA	3566199..3567294 (+)	1096	Protein_3572	DNA polymerase III subunit delta	-
LGL65_RS18385	rpsT	3567257..3567523 (-)	267	WP_003152876.1	30S ribosomal protein S20	-
LGL65_RS18390	gpr	3567726..3568832 (+)	1107	WP_003152878.1	GPR endopeptidase	-
LGL65_RS18395	-	3568900..3570093 (+)	1194	WP_007612673.1	stage II sporulation protein P	-
LGL65_RS18400	-	3570110..3570448 (+)	339	WP_017418178.1	YqxA family protein	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86725.67 Da
• Isoelectric Point: 8.9055

>NTDB_id=655988 LGL65_RS18365 WP_025649830.1 3563463..3565814(+) (comEC) [Bacillus velezensis strain LOH112]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QVVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSGQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSV
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEEIRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=655988 LGL65_RS18365 WP_025649830.1 3563463..3565814(+) (comEC) [Bacillus velezensis strain LOH112]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGTCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAGGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTACATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACTCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGTT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAATCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-469)

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

3D structure

• AlphaFold DB: A0A411A869

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	57.05	98.723	0.563