Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L8M16_RS17115 Genome accession   NZ_CP091977
Coordinates   3576806..3578035 (-) Length   409 a.a.
NCBI ID   WP_011236570.1    Uniprot ID   Q5P769
Organism   Aromatoleum aromaticum strain pCyN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3571806..3583035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS17085 - 3572294..3573289 (+) 996 WP_168953464.1 SIS domain-containing protein -
  L8M16_RS17090 - 3573289..3573810 (+) 522 WP_168953463.1 HAD family hydrolase -
  L8M16_RS17095 lptC 3573819..3574400 (+) 582 WP_168953462.1 LPS export ABC transporter periplasmic protein LptC -
  L8M16_RS17100 lptA 3574397..3574969 (+) 573 WP_011236566.1 lipopolysaccharide transport periplasmic protein LptA -
  L8M16_RS17105 - 3575020..3575796 (+) 777 WP_011236567.1 enoyl-CoA hydratase -
  L8M16_RS17110 - 3576148..3576708 (+) 561 WP_011236569.1 phasin family protein -
  L8M16_RS17115 pilU 3576806..3578035 (-) 1230 WP_011236570.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L8M16_RS17120 - 3578144..3578935 (-) 792 WP_011236571.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  L8M16_RS17125 gmhB 3578944..3579477 (-) 534 WP_011236572.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  L8M16_RS17130 glyS 3579493..3581580 (-) 2088 WP_168953461.1 glycine--tRNA ligase subunit beta -
  L8M16_RS17135 glyQ 3581580..3582500 (-) 921 WP_041645683.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45089.43 Da        Isoelectric Point: 6.4035

>NTDB_id=654828 L8M16_RS17115 WP_011236570.1 3576806..3578035(-) (pilU) [Aromatoleum aromaticum strain pCyN1]
MASALGPEEARNYMHKLLKAMHQMGGSDLFISADFPPSIKSHGGMKPLSQQRLAAEVTKSLALSLMNERQRREFEAEMEC
NFAVSLPDVCRFRVNVFIQQQQVGMVIRTIASEIPNLDKLDLPGVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNT
NSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWEHALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNSNQTLDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGAGRKAAIEILLNTPTIAEMIFKGQFHSIKEIMAKS
RELGMCTFDQALFALYDAHHISYEEAIRNADSANELRLQIKLKAGRGQPSSGIGSEFSLSIEESVEEDSGEAEKAELLPA
SGASTAHTS

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=654828 L8M16_RS17115 WP_011236570.1 3576806..3578035(-) (pilU) [Aromatoleum aromaticum strain pCyN1]
ATGGCATCCGCGCTCGGCCCTGAAGAAGCACGCAATTACATGCACAAGCTGCTGAAGGCGATGCACCAGATGGGAGGCTC
GGACCTTTTCATCTCGGCCGACTTCCCGCCGAGCATCAAGTCGCACGGCGGCATGAAACCGCTGAGCCAGCAGCGGCTCG
CTGCCGAAGTGACCAAGTCGCTCGCGCTGAGCCTGATGAACGAGCGCCAGCGGCGCGAATTCGAGGCCGAGATGGAGTGC
AATTTCGCCGTCTCGCTGCCCGACGTGTGCCGCTTCCGCGTCAACGTGTTCATCCAGCAACAGCAGGTCGGCATGGTGAT
CCGGACGATCGCCTCGGAAATTCCGAATCTCGACAAGCTCGACCTGCCCGGCGTGCTCAAGGACGTCATCATGACGAAGC
GCGGTCTGGTGCTTGTCGTGGGCGGCACGGGATCGGGCAAGTCGACGACACTGGCGGCGATGATCGACCACCGCAACACG
AATTCGGCCGGCCACATCATCACCGTCGAGGATCCGGTCGAGTACGTGCACAAGAACAAGGGCTGCCTCGTCACTCACCG
TGAAGTCGGCGTCGATACGCATTCTTGGGAGCACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGATCGCGAGACGATGGAGCACGCCATTGCATTTGCCGAAACCGGGCACCTGTGTCTGGGGACGCTGCAC
GCGAACAATTCGAATCAGACGCTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCAACCAGCTCCTGATGGACCT
TTCGTCGAACCTGCGGGCGATCGTCTCGCAGCGCCTCGTCCGCACCGAGGACGGCGCCGGGCGCAAGGCCGCGATCGAAA
TCCTCCTGAACACCCCGACGATCGCCGAAATGATCTTCAAGGGACAGTTCCATTCGATCAAGGAGATCATGGCGAAGTCG
CGCGAACTGGGGATGTGCACGTTCGATCAGGCGCTGTTCGCCCTGTATGACGCGCACCACATCAGCTACGAAGAGGCGAT
CCGGAATGCCGACTCGGCGAACGAGTTGCGCCTGCAGATCAAGCTGAAGGCCGGCCGCGGGCAGCCGTCGTCGGGCATTG
GCAGCGAGTTCAGCCTGTCGATCGAGGAGTCCGTGGAGGAGGATTCAGGCGAAGCGGAAAAAGCGGAGCTGCTGCCCGCG
TCTGGCGCCTCGACGGCACACACGTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P769

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.942

84.841

0.509
  pilU Acinetobacter baylyi ADP1

55.085

86.553

0.477
  pilU Vibrio cholerae strain A1552

51.136

86.064

0.44
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.662

86.797

0.379