Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EBS_RS12325 Genome accession   NZ_AP012978
Coordinates   2546787..2547863 (-) Length   358 a.a.
NCBI ID   WP_148307762.1    Uniprot ID   -
Organism   Endosymbiont of unidentified scaly snail isolate Monju     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2541787..2552863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBS_RS12300 (EBS_2354) aroK 2542399..2542911 (-) 513 WP_043108939.1 shikimate kinase AroK -
  EBS_RS12305 (EBS_2355) pilQ 2542985..2545090 (-) 2106 WP_052199580.1 type IV pilus secretin PilQ -
  EBS_RS12310 (EBS_2356) - 2545104..2545622 (-) 519 WP_043108941.1 pilus assembly protein PilP -
  EBS_RS12315 - 2545622..2546230 (-) 609 WP_043108942.1 type 4a pilus biogenesis protein PilO -
  EBS_RS12320 (EBS_2358) - 2546134..2546787 (-) 654 WP_070104741.1 PilN domain-containing protein -
  EBS_RS12325 (EBS_2359) pilM 2546787..2547863 (-) 1077 WP_148307762.1 pilus assembly protein PilM Machinery gene
  EBS_RS12330 (EBS_2360) - 2548125..2550536 (+) 2412 WP_148307763.1 penicillin-binding protein 1A -
  EBS_RS12335 (EBS_2361) - 2550563..2551864 (-) 1302 WP_043108944.1 citrate synthase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38890.42 Da        Isoelectric Point: 4.4907

>NTDB_id=65418 EBS_RS12325 WP_148307762.1 2546787..2547863(-) (pilM) [Endosymbiont of unidentified scaly snail isolate Monju]
MMGLFSRKKPLMLGLDISSTAVKLLELSQHSGRGGVRYRVESYAVEPLPPSAVVEKNIADVEAVGETIRKTLKRAGTRTR
HAAVAVSGSAVITKVITLPASMSEREMETQIELEADQHIPYPLEEVNIDFQILGPSEKSPEMVDVLLAASRSENVDARVE
ALDLAGLICEVVDVEAYAMERACGQLADQWPNGGEDLVVAVADIGATTTTLDVLYDNRVIYTREQNFGGRQLTEEIQRRY
GLSMEEAGMAKRQGGLPDNYIPEVLEPFREAMVQQVNRSIQFFYSASNHNAVDLIVLAGGCASVAGIEDLVQERLGIETM
IANPLANMSVGPRVKPQALSNDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=65418 EBS_RS12325 WP_148307762.1 2546787..2547863(-) (pilM) [Endosymbiont of unidentified scaly snail isolate Monju]
CTGATGGGACTGTTCTCCAGAAAGAAGCCTCTCATGCTGGGGCTGGATATCAGCTCCACGGCCGTCAAGTTGCTGGAACT
CAGTCAGCACAGCGGGCGTGGAGGTGTGCGTTACCGCGTCGAGAGCTATGCCGTGGAGCCGCTACCCCCCTCCGCCGTGG
TGGAAAAGAACATCGCGGATGTCGAGGCCGTGGGCGAAACCATCCGCAAGACGCTCAAGCGGGCCGGCACTCGCACCCGG
CATGCGGCGGTCGCGGTTTCCGGTTCGGCGGTCATCACCAAGGTCATCACCCTGCCGGCCTCCATGTCCGAGCGCGAGAT
GGAGACCCAGATCGAACTCGAGGCCGATCAGCACATCCCCTACCCGCTCGAGGAAGTGAACATCGACTTCCAGATCCTCG
GGCCCTCGGAGAAGAGCCCCGAGATGGTCGATGTGCTGCTGGCCGCCTCGCGCAGCGAGAACGTCGATGCCCGGGTCGAG
GCGCTGGACCTGGCCGGGCTGATCTGCGAAGTGGTGGACGTCGAGGCCTATGCCATGGAGCGGGCCTGCGGCCAGCTCGC
CGACCAGTGGCCCAACGGTGGCGAAGACCTGGTGGTGGCGGTGGCGGACATCGGCGCCACCACGACCACCCTCGATGTGC
TCTACGACAATCGCGTCATCTACACCCGTGAACAGAACTTCGGTGGACGTCAGCTCACCGAGGAGATCCAGCGCCGCTAC
GGCCTGTCGATGGAAGAAGCTGGCATGGCCAAGCGCCAGGGCGGCCTGCCCGACAACTACATCCCGGAGGTACTCGAACC
CTTCCGCGAGGCCATGGTGCAGCAGGTCAATCGTTCCATCCAGTTCTTCTATTCGGCCAGCAACCACAATGCCGTGGACC
TGATCGTGCTGGCAGGTGGCTGTGCCTCGGTGGCCGGCATCGAGGACCTGGTGCAGGAACGACTGGGCATAGAGACCATG
ATTGCCAATCCCCTGGCCAACATGTCGGTGGGCCCCCGGGTCAAGCCGCAGGCCCTCAGTAACGATGCCCCCGCGATGAT
GATCGCCTGTGGCCTTGCCCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.838

100

0.508
  comM Acinetobacter nosocomialis M2

50.559

100

0.506
  comM Acinetobacter baylyi ADP1

48.883

100

0.489
  pilM Legionella pneumophila strain ERS1305867

46.369

100

0.464

Multiple sequence alignment