Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MAK48_RS12560 Genome accession   NZ_CP091872
Coordinates   2451545..2452615 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus sp. Man122     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2446545..2457615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAK48_RS12515 (MAK48_12515) tapA 2447024..2447785 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  MAK48_RS12520 (MAK48_12520) - 2448057..2448383 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  MAK48_RS12525 (MAK48_12525) - 2448425..2448604 (-) 180 WP_003230176.1 YqzE family protein -
  MAK48_RS12530 (MAK48_12530) comGG 2448675..2449049 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  MAK48_RS12535 (MAK48_12535) comGF 2449050..2449433 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  MAK48_RS12540 (MAK48_12540) comGE 2449459..2449806 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  MAK48_RS12545 (MAK48_12545) comGD 2449790..2450221 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  MAK48_RS12550 (MAK48_12550) comGC 2450211..2450507 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  MAK48_RS12555 (MAK48_12555) comGB 2450521..2451558 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  MAK48_RS12560 (MAK48_12560) comGA 2451545..2452615 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  MAK48_RS21235 - 2452826..2452951 (-) 126 WP_003230155.1 hypothetical protein -
  MAK48_RS12565 (MAK48_12565) corA 2453017..2453970 (-) 954 WP_032677123.1 magnesium transporter CorA -
  MAK48_RS12570 (MAK48_12570) - 2454113..2455441 (+) 1329 WP_003230150.1 hemolysin family protein -
  MAK48_RS12575 (MAK48_12575) rsbRD 2455494..2456330 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  MAK48_RS12585 (MAK48_12585) mgsR 2456554..2456934 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  MAK48_RS12590 (MAK48_12590) - 2457166..2457411 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=654039 MAK48_RS12560 WP_004399124.1 2451545..2452615(-) (comGA) [Bacillus sp. Man122]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=654039 MAK48_RS12560 WP_004399124.1 2451545..2452615(-) (comGA) [Bacillus sp. Man122]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1