Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L3Q70_RS03200 Genome accession   NZ_CP091442
Coordinates   675266..676399 (+) Length   377 a.a.
NCBI ID   WP_237114835.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. CF6-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 670266..681399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Q70_RS03170 (L3Q70_03170) - 670997..671596 (-) 600 WP_062570112.1 XTP/dITP diphosphatase -
  L3Q70_RS03175 (L3Q70_03175) - 671604..672044 (-) 441 WP_237114833.1 DUF4426 domain-containing protein -
  L3Q70_RS03180 (L3Q70_03180) - 672099..672632 (-) 534 WP_062570114.1 YggT family protein -
  L3Q70_RS03185 (L3Q70_03185) proC 672656..673477 (-) 822 WP_062570115.1 pyrroline-5-carboxylate reductase -
  L3Q70_RS03190 (L3Q70_03190) - 673495..674208 (-) 714 WP_237114834.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3Q70_RS03195 (L3Q70_03195) pilT 674211..675251 (+) 1041 WP_062570117.1 type IV pilus twitching motility protein PilT Machinery gene
  L3Q70_RS03200 (L3Q70_03200) pilU 675266..676399 (+) 1134 WP_237114835.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L3Q70_RS03205 (L3Q70_03205) - 676399..677268 (+) 870 WP_237114836.1 hypothetical protein -
  L3Q70_RS03210 (L3Q70_03210) ruvX 677265..677714 (-) 450 WP_193988351.1 Holliday junction resolvase RuvX -
  L3Q70_RS03215 (L3Q70_03215) - 677745..678302 (-) 558 WP_062570121.1 YqgE/AlgH family protein -
  L3Q70_RS03220 (L3Q70_03220) gshB 678327..679280 (-) 954 WP_237114837.1 glutathione synthase -
  L3Q70_RS03225 (L3Q70_03225) rsmE 679349..680083 (-) 735 WP_062570123.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3Q70_RS03230 (L3Q70_03230) - 680143..680607 (-) 465 WP_062570124.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41774.71 Da        Isoelectric Point: 5.9727

>NTDB_id=651163 L3Q70_RS03200 WP_237114835.1 675266..676399(+) (pilU) [Pseudoalteromonas sp. CF6-2]
MTIPLNRFLQVMIDKNGSDLFVSSGLSVSAKINGELQPLTDDKLSDEQALAMVESAMNEKQRNEFHATKECNFAIATEEG
RFRVSAFWQRDQAGMVIRRIVTQIPDVNDLGLPSILTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGEGRVAAIEILLNSPMVAELIKRGDIGSIKETMAKSKEMGMQTFDQ
ALFELYKHQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTIDGLDEKGNS

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=651163 L3Q70_RS03200 WP_237114835.1 675266..676399(+) (pilU) [Pseudoalteromonas sp. CF6-2]
ATGACAATACCTTTAAATCGCTTTTTACAGGTGATGATCGATAAGAATGGTTCAGATTTATTTGTGTCTAGTGGCTTATC
TGTTAGTGCCAAAATTAACGGTGAATTGCAACCGCTGACCGACGATAAGTTATCTGATGAGCAGGCACTGGCTATGGTTG
AGTCTGCGATGAACGAAAAGCAACGTAATGAGTTTCACGCAACAAAAGAATGTAACTTTGCCATTGCAACAGAAGAAGGG
CGGTTTCGTGTTTCTGCATTTTGGCAGCGAGACCAAGCGGGTATGGTTATTCGCCGAATTGTGACGCAAATTCCTGATGT
AAACGATTTAGGATTACCGTCTATTCTTACCGATGTGATAATGGCAAAACGTGGCTTAGTGCTGTTTGTAGGTGGTACCG
GTACCGGTAAGTCAACCTCGCTTGCTGCGCTTTTAGGTTATCGAAATCGTAATCAACGTGGGCATATTCTTACCATTGAA
GATCCAATCGAGTTTGTGCATGAGCACCAAAAAAGTATTATTACTCAACGCGAAGTAGGTCTTGATACTGAAAGCTTTGA
GTCAGCGTTAAAAAGCTCATTACGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATCCGTTCACAAGAAACCATGGAAT
ATGCATTAAGTTTTGCTGAGACAGGGCATTTATGTGTCGCAACGTTGCACGCTAATAATGCCAACCAAGCCATTGACCGT
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTAAAATACGACTTAGCACTGAACCTACGCGCAATTGTTGCACA
GCAACTGGTGCCAACAGCGGATGGGGAAGGCCGTGTGGCTGCTATCGAGATCTTGCTTAATTCTCCTATGGTCGCTGAAC
TGATAAAGCGCGGTGATATTGGTTCAATAAAAGAAACTATGGCGAAATCAAAAGAAATGGGTATGCAGACTTTTGACCAA
GCCTTGTTTGAGCTTTATAAACACCAGCGTATTAATTATGCCGATGCCTTGCATCATGCTGACTCTCCAAACGATTTACG
CTTGATGATCAAGTTACAAAATAACGAGCAAAAAGGGGCTGGCTTTTTACAAGGTGTGACTATTGATGGCCTTGATGAAA
AGGGTAATAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.568

98.143

0.565
  pilU Acinetobacter baylyi ADP1

56.533

99.469

0.562
  pilU Vibrio cholerae strain A1552

56.131

97.347

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.778

95.491

0.408