Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   L3I92_RS04985 Genome accession   NZ_CP091412
Coordinates   942806..943303 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain ATR-20003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937806..948303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3I92_RS04945 (L3I92_000989) - 937850..938836 (+) 987 WP_111187437.1 ROK family glucokinase -
  L3I92_RS04950 (L3I92_000990) - 938836..939165 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  L3I92_RS04955 (L3I92_000991) - 939162..939785 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  L3I92_RS04960 (L3I92_000992) comGA 939837..940811 (+) 975 WP_000697223.1 competence type IV pilus ATPase ComGA Machinery gene
  L3I92_RS04965 (L3I92_000993) comGB 940783..941853 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  L3I92_RS04970 (L3I92_000994) comGC 941867..942178 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  L3I92_RS04975 (L3I92_000995) comGD 942156..942602 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  L3I92_RS04980 (L3I92_000996) comGE 942589..942888 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  L3I92_RS04985 (L3I92_000997) comGF 942806..943303 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  L3I92_RS04990 (L3I92_000998) - 943400..943546 (+) 147 WP_001789879.1 hypothetical protein -
  L3I92_RS04995 (L3I92_000999) - 943536..944060 (+) 525 WP_001015120.1 shikimate kinase -
  L3I92_RS05000 (L3I92_001000) gcvT 944219..945310 (+) 1092 WP_237269230.1 glycine cleavage system aminomethyltransferase GcvT -
  L3I92_RS05005 (L3I92_001001) gcvPA 945330..946676 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  L3I92_RS05010 (L3I92_001002) gcvPB 946669..948141 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=650882 L3I92_RS04985 WP_001796472.1 942806..943303(+) (comGF) [Staphylococcus aureus strain ATR-20003]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=650882 L3I92_RS04985 WP_001796472.1 942806..943303(+) (comGF) [Staphylococcus aureus strain ATR-20003]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958