Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L3I92_RS04960 Genome accession   NZ_CP091412
Coordinates   939837..940811 (+) Length   324 a.a.
NCBI ID   WP_000697223.1    Uniprot ID   -
Organism   Staphylococcus aureus strain ATR-20003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934837..945811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3I92_RS04925 (L3I92_000985) rpmG 935297..935446 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  L3I92_RS04930 (L3I92_000986) - 935655..936194 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  L3I92_RS04935 (L3I92_000987) - 936206..937669 (+) 1464 WP_001017870.1 rhomboid family intramembrane serine protease -
  L3I92_RS04940 (L3I92_000988) - 937650..937853 (+) 204 WP_000087561.1 YqgQ family protein -
  L3I92_RS04945 (L3I92_000989) - 937850..938836 (+) 987 WP_111187437.1 ROK family glucokinase -
  L3I92_RS04950 (L3I92_000990) - 938836..939165 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  L3I92_RS04955 (L3I92_000991) - 939162..939785 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  L3I92_RS04960 (L3I92_000992) comGA 939837..940811 (+) 975 WP_000697223.1 competence type IV pilus ATPase ComGA Machinery gene
  L3I92_RS04965 (L3I92_000993) comGB 940783..941853 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  L3I92_RS04970 (L3I92_000994) comGC 941867..942178 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  L3I92_RS04975 (L3I92_000995) comGD 942156..942602 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  L3I92_RS04980 (L3I92_000996) comGE 942589..942888 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  L3I92_RS04985 (L3I92_000997) comGF 942806..943303 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  L3I92_RS04990 (L3I92_000998) - 943400..943546 (+) 147 WP_001789879.1 hypothetical protein -
  L3I92_RS04995 (L3I92_000999) - 943536..944060 (+) 525 WP_001015120.1 shikimate kinase -
  L3I92_RS05000 (L3I92_001000) gcvT 944219..945310 (+) 1092 WP_237269230.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36926.01 Da        Isoelectric Point: 8.8516

>NTDB_id=650877 L3I92_RS04960 WP_000697223.1 939837..940811(+) (comGA) [Staphylococcus aureus strain ATR-20003]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQVTNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=650877 L3I92_RS04960 WP_000697223.1 939837..940811(+) (comGA) [Staphylococcus aureus strain ATR-20003]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGTAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997