Detailed information NTG0650517

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: L2Z09_RS16625
• Genome accession: NZ_CP091365
• Coordinates: 3463512..3464570 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AB172-VUB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3458512..3469570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z09_RS16600 (L2Z09_16595)	aroK	3458782..3459351 (-)	570	WP_000643712.1	shikimate kinase AroK	-
L2Z09_RS16605 (L2Z09_16600)	pilQ	3459363..3461528 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
L2Z09_RS16610 (L2Z09_16605)	pilP	3461596..3462123 (-)	528	WP_215745106.1	pilus assembly protein PilP	Machinery gene
L2Z09_RS16615 (L2Z09_16610)	pilO	3462134..3462874 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
L2Z09_RS16620 (L2Z09_16615)	pilN	3462871..3463512 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
L2Z09_RS16625 (L2Z09_16620)	pilM	3463512..3464570 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
L2Z09_RS16630 (L2Z09_16625)	ponA	3464733..3467288 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
L2Z09_RS16635 (L2Z09_16630)	-	3467306..3468130 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
L2Z09_RS16640 (L2Z09_16635)	-	3468133..3468465 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=650517 L2Z09_RS16625 WP_002014066.1 3463512..3464570(-) (pilM) [Acinetobacter baumannii strain AB172-VUB]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=650517 L2Z09_RS16625 WP_002014066.1 3463512..3464570(-) (pilM) [Acinetobacter baumannii strain AB172-VUB]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423