Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Z09_RS03495 Genome accession   NZ_CP091365
Coordinates   737814..738932 (-) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain AB172-VUB     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 719915..780933 737814..738932 within 0


Gene organization within MGE regions


Location: 719915..780933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Z09_RS03395 (L2Z09_03395) - 720140..720784 (-) 645 WP_047936530.1 tyrosyl-tRNA synthetase -
  L2Z09_RS03405 (L2Z09_03405) - 722121..722408 (-) 288 WP_001218560.1 PA4642 family protein -
  L2Z09_RS03410 (L2Z09_03410) minE 722492..722764 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  L2Z09_RS03415 (L2Z09_03415) minD 722767..723579 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  L2Z09_RS03420 (L2Z09_03420) minC 723650..724372 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  L2Z09_RS03425 (L2Z09_03425) - 724494..725540 (-) 1047 WP_001181639.1 hypothetical protein -
  L2Z09_RS03430 (L2Z09_03430) - 725557..726483 (-) 927 WP_000100965.1 acyltransferase -
  L2Z09_RS03435 (L2Z09_03435) - 726728..727381 (+) 654 WP_001202415.1 OmpA family protein -
  L2Z09_RS03440 (L2Z09_03440) rep 727575..729614 (+) 2040 WP_000093035.1 DNA helicase Rep -
  L2Z09_RS03445 (L2Z09_03445) dut 729639..730091 (+) 453 WP_000868152.1 dUTP diphosphatase -
  L2Z09_RS03450 (L2Z09_03450) - 730290..731708 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  L2Z09_RS03455 (L2Z09_03455) argB 731723..732631 (+) 909 WP_001135419.1 acetylglutamate kinase -
  L2Z09_RS03460 (L2Z09_03460) - 732752..733534 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  L2Z09_RS03465 (L2Z09_03465) - 733643..734491 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  L2Z09_RS03470 (L2Z09_03470) - 734744..735199 (+) 456 WP_000782976.1 bacteriohemerythrin -
  L2Z09_RS03475 (L2Z09_03475) - 735352..736419 (+) 1068 WP_002019572.1 hypothetical protein -
  L2Z09_RS03480 (L2Z09_03480) - 736419..736742 (+) 324 WP_000442587.1 RnfH family protein -
  L2Z09_RS03485 (L2Z09_03485) bamE 736797..737195 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  L2Z09_RS03490 (L2Z09_03490) fur 737307..737744 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  L2Z09_RS03495 (L2Z09_03495) pilU 737814..738932 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Z09_RS03500 (L2Z09_03500) pilT 738960..739997 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Z09_RS03505 (L2Z09_03505) - 740124..740816 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2Z09_RS03510 (L2Z09_03510) - 741018..744614 (-) 3597 WP_215745102.1 AAA family ATPase -
  L2Z09_RS03515 (L2Z09_03515) - 744624..745892 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  L2Z09_RS03520 (L2Z09_03520) - 746056..746529 (+) 474 WP_000974358.1 OsmC family protein -
  L2Z09_RS03525 (L2Z09_03525) - 746597..746974 (-) 378 WP_001059438.1 VOC family protein -
  L2Z09_RS03530 (L2Z09_03530) - 747354..747806 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  L2Z09_RS03535 (L2Z09_03535) - 747896..748801 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  L2Z09_RS03540 (L2Z09_03540) - 748793..749908 (-) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  L2Z09_RS03545 (L2Z09_03545) hemB 749934..750947 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  L2Z09_RS03550 (L2Z09_03550) - 751090..751587 (+) 498 WP_002019577.1 thioesterase family protein -
  L2Z09_RS03555 (L2Z09_03555) - 751857..753008 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  L2Z09_RS03560 (L2Z09_03560) - 753015..754538 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  L2Z09_RS03565 (L2Z09_03565) ggt 754841..756826 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  L2Z09_RS03575 (L2Z09_03575) - 757528..758757 (+) 1230 WP_000831316.1 tyrosine-type recombinase/integrase -
  L2Z09_RS03580 (L2Z09_03580) - 758905..759672 (+) 768 WP_000578616.1 hypothetical protein -
  L2Z09_RS03585 (L2Z09_03585) - 759882..760097 (+) 216 WP_000153154.1 hypothetical protein -
  L2Z09_RS03590 (L2Z09_03590) - 760100..760309 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  L2Z09_RS03595 (L2Z09_03595) - 760306..760860 (+) 555 WP_001988245.1 BRO family protein -
  L2Z09_RS03600 (L2Z09_03600) - 760853..761221 (+) 369 WP_001046481.1 hypothetical protein -
  L2Z09_RS03605 (L2Z09_03605) - 761231..761410 (+) 180 Protein_688 hypothetical protein -
  L2Z09_RS03610 (L2Z09_03610) - 761441..762283 (-) 843 WP_225468757.1 IS3 family transposase -
  L2Z09_RS03615 (L2Z09_03615) - 762316..762824 (-) 509 Protein_690 transposase -
  L2Z09_RS03620 (L2Z09_03620) - 763127..763405 (+) 279 WP_001064707.1 hypothetical protein -
  L2Z09_RS03625 (L2Z09_03625) - 763418..765883 (+) 2466 WP_001022396.1 DUF927 domain-containing protein -
  L2Z09_RS03630 (L2Z09_03630) - 766216..766419 (+) 204 WP_001984265.1 single-stranded DNA-binding protein -
  L2Z09_RS03635 (L2Z09_03635) - 766556..767998 (+) 1443 WP_000003532.1 hypothetical protein -
  L2Z09_RS03640 (L2Z09_03640) - 768341..769015 (+) 675 WP_000560651.1 DUF3800 domain-containing protein -
  L2Z09_RS03645 (L2Z09_03645) - 769015..769203 (+) 189 WP_000642900.1 hypothetical protein -
  L2Z09_RS03650 (L2Z09_03650) - 769259..769924 (+) 666 WP_000515964.1 hypothetical protein -
  L2Z09_RS03655 (L2Z09_03655) - 772624..773667 (+) 1044 WP_240367488.1 AAA family ATPase -
  L2Z09_RS03660 (L2Z09_03660) - 773664..774245 (+) 582 WP_025468727.1 hypothetical protein -
  L2Z09_RS03665 (L2Z09_03665) - 774310..775173 (+) 864 WP_000109893.1 DNA adenine methylase -
  L2Z09_RS03670 (L2Z09_03670) - 775564..776514 (+) 951 WP_000998814.1 hypothetical protein -
  L2Z09_RS03675 (L2Z09_03675) - 776519..776821 (-) 303 WP_000020972.1 hypothetical protein -
  L2Z09_RS03680 (L2Z09_03680) - 777102..777386 (-) 285 WP_000675273.1 hypothetical protein -
  L2Z09_RS03685 (L2Z09_03685) - 778033..779379 (-) 1347 WP_001046209.1 OprD family outer membrane porin -
  L2Z09_RS03690 (L2Z09_03690) - 779457..780842 (-) 1386 WP_000182788.1 MFS transporter -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=650490 L2Z09_RS03495 WP_000347039.1 737814..738932(-) (pilU) [Acinetobacter baumannii strain AB172-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=650490 L2Z09_RS03495 WP_000347039.1 737814..738932(-) (pilU) [Acinetobacter baumannii strain AB172-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395