Detailed information NTG0649786

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Z31_RS03305
• Genome accession: NZ_CP091345
• Coordinates: 698545..699663 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AB219-VUB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 693545..704663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z31_RS03275 (L2Z31_03275)	-	694374..695222 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
L2Z31_RS03280 (L2Z31_03280)	-	695475..695930 (+)	456	WP_000782976.1	bacteriohemerythrin	-
L2Z31_RS03285 (L2Z31_03285)	-	696083..697150 (+)	1068	WP_002019572.1	hypothetical protein	-
L2Z31_RS03290 (L2Z31_03290)	-	697150..697473 (+)	324	WP_000442587.1	RnfH family protein	-
L2Z31_RS03295 (L2Z31_03295)	bamE	697528..697926 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
L2Z31_RS03300 (L2Z31_03300)	fur	698038..698475 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
L2Z31_RS03305 (L2Z31_03305)	pilU	698545..699663 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Z31_RS03310 (L2Z31_03310)	pilT	699691..700728 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
L2Z31_RS03315 (L2Z31_03315)	-	700855..701547 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=649786 L2Z31_RS03305 WP_000347039.1 698545..699663(-) (pilU) [Acinetobacter baumannii strain AB219-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=649786 L2Z31_RS03305 WP_000347039.1 698545..699663(-) (pilU) [Acinetobacter baumannii strain AB219-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395