Detailed information NTG0649519

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Z38_RS14005
• Genome accession: NZ_CP091339
• Coordinates: 2938757..2939875 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB229-VUB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2933757..2944875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z38_RS13995 (L2Z38_13995)	-	2936873..2937565 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
L2Z38_RS14000 (L2Z38_14000)	pilT	2937692..2938729 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
L2Z38_RS14005 (L2Z38_14005)	pilU	2938757..2939875 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Z38_RS14010 (L2Z38_14010)	fur	2939945..2940382 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
L2Z38_RS14015 (L2Z38_14015)	bamE	2940494..2940892 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
L2Z38_RS14020 (L2Z38_14020)	-	2940947..2941270 (-)	324	WP_000442585.1	RnfH family protein	-
L2Z38_RS14025 (L2Z38_14025)	-	2941270..2942337 (-)	1068	WP_000092403.1	hypothetical protein	-
L2Z38_RS14030 (L2Z38_14030)	-	2942490..2942945 (-)	456	WP_000782976.1	bacteriohemerythrin	-
L2Z38_RS14035 (L2Z38_14035)	-	2943198..2944046 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=649519 L2Z38_RS14005 WP_000347036.1 2938757..2939875(+) (pilU) [Acinetobacter baumannii strain AB229-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=649519 L2Z38_RS14005 WP_000347036.1 2938757..2939875(+) (pilU) [Acinetobacter baumannii strain AB229-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392