Detailed information NTG0649357

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Z42_RS08555
• Genome accession: NZ_CP091335
• Coordinates: 1750540..1751658 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ATCC17978-VUB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1745540..1756658

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z42_RS08545 (L2Z42_08545)	-	1748656..1749348 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
L2Z42_RS08550 (L2Z42_08550)	pilT	1749475..1750512 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
L2Z42_RS08555 (L2Z42_08555)	pilU	1750540..1751658 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Z42_RS08560 (L2Z42_08560)	fur	1751728..1752165 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
L2Z42_RS08565 (L2Z42_08565)	bamE	1752277..1752675 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
L2Z42_RS08570 (L2Z42_08570)	-	1752730..1753053 (-)	324	WP_000442590.1	RnfH family protein	-
L2Z42_RS08575 (L2Z42_08575)	-	1753053..1754120 (-)	1068	WP_004748097.1	hypothetical protein	-
L2Z42_RS08580 (L2Z42_08580)	-	1754273..1754728 (-)	456	WP_000782976.1	bacteriohemerythrin	-
L2Z42_RS08585 (L2Z42_08585)	-	1754981..1755829 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=649357 L2Z42_RS08555 WP_000347034.1 1750540..1751658(+) (pilU) [Acinetobacter baumannii strain ATCC17978-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=649357 L2Z42_RS08555 WP_000347034.1 1750540..1751658(+) (pilU) [Acinetobacter baumannii strain ATCC17978-VUB]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39