Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Z43_RS16160 Genome accession   NZ_CP091334
Coordinates   3404721..3405839 (-) Length   372 a.a.
NCBI ID   WP_000347036.1    Uniprot ID   A0A009PAD6
Organism   Acinetobacter baumannii strain ATCC19606-VUB     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3369262..3436414 3404721..3405839 within 0


Gene organization within MGE regions


Location: 3369262..3436414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Z43_RS15985 (L2Z43_15985) - 3369663..3370223 (-) 561 WP_001048354.1 hypothetical protein -
  L2Z43_RS15990 (L2Z43_15990) - 3370314..3371174 (-) 861 WP_000838088.1 pirin family protein -
  L2Z43_RS15995 (L2Z43_15995) hemJ 3371345..3371797 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  L2Z43_RS16000 (L2Z43_16000) - 3371816..3373045 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  L2Z43_RS16005 (L2Z43_16005) - 3373342..3374667 (+) 1326 WP_000160010.1 EcsC family protein -
  L2Z43_RS16010 (L2Z43_16010) rpsL 3374868..3375242 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  L2Z43_RS16015 (L2Z43_16015) rpsG 3375402..3375872 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  L2Z43_RS16020 (L2Z43_16020) fusA 3376051..3378189 (+) 2139 WP_000113824.1 elongation factor G -
  L2Z43_RS16025 (L2Z43_16025) tuf 3378284..3379474 (+) 1191 WP_001029610.1 elongation factor Tu -
  L2Z43_RS16030 (L2Z43_16030) - 3379678..3380559 (+) 882 WP_000992301.1 metal-dependent hydrolase -
  L2Z43_RS16035 (L2Z43_16035) - 3380796..3381671 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  L2Z43_RS16040 (L2Z43_16040) rimI 3381779..3382234 (+) 456 WP_000621546.1 ribosomal protein S18-alanine N-acetyltransferase -
  L2Z43_RS16045 (L2Z43_16045) - 3382279..3383094 (-) 816 WP_000844343.1 arginyltransferase -
  L2Z43_RS16050 (L2Z43_16050) aat 3383113..3383844 (-) 732 WP_000854789.1 leucyl/phenylalanyl-tRNA--protein transferase -
  L2Z43_RS16055 (L2Z43_16055) trxB 3383965..3384912 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  L2Z43_RS16060 (L2Z43_16060) - 3385182..3388214 (+) 3033 WP_000127835.1 DNA translocase FtsK -
  L2Z43_RS16065 (L2Z43_16065) rhtC 3388363..3388983 (+) 621 WP_000959259.1 threonine export protein RhtC -
  L2Z43_RS16070 (L2Z43_16070) - 3389026..3389313 (-) 288 WP_001218560.1 PA4642 family protein -
  L2Z43_RS16075 (L2Z43_16075) minE 3389397..3389669 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  L2Z43_RS16080 (L2Z43_16080) minD 3389672..3390484 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  L2Z43_RS16085 (L2Z43_16085) minC 3390555..3391277 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  L2Z43_RS16090 (L2Z43_16090) - 3391399..3392445 (-) 1047 WP_001181637.1 hypothetical protein -
  L2Z43_RS16095 (L2Z43_16095) - 3392462..3393388 (-) 927 WP_000100975.1 acyltransferase -
  L2Z43_RS16100 (L2Z43_16100) - 3393633..3394286 (+) 654 WP_001202415.1 OmpA family protein -
  L2Z43_RS16105 (L2Z43_16105) rep 3394480..3396519 (+) 2040 WP_000093035.1 DNA helicase Rep -
  L2Z43_RS16110 (L2Z43_16110) dut 3396545..3396997 (+) 453 WP_000868152.1 dUTP diphosphatase -
  L2Z43_RS16115 (L2Z43_16115) - 3397197..3398615 (+) 1419 WP_001102833.1 phosphomannomutase/phosphoglucomutase -
  L2Z43_RS16120 (L2Z43_16120) argB 3398630..3399538 (+) 909 WP_001135419.1 acetylglutamate kinase -
  L2Z43_RS16125 (L2Z43_16125) - 3399659..3400441 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  L2Z43_RS16130 (L2Z43_16130) - 3400550..3401398 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  L2Z43_RS16135 (L2Z43_16135) - 3401651..3402106 (+) 456 WP_000782976.1 bacteriohemerythrin -
  L2Z43_RS16140 (L2Z43_16140) - 3402259..3403326 (+) 1068 WP_005144332.1 hypothetical protein -
  L2Z43_RS16145 (L2Z43_16145) - 3403326..3403649 (+) 324 WP_000442584.1 RnfH family protein -
  L2Z43_RS16150 (L2Z43_16150) bamE 3403704..3404102 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  L2Z43_RS16155 (L2Z43_16155) fur 3404214..3404651 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  L2Z43_RS16160 (L2Z43_16160) pilU 3404721..3405839 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Z43_RS16165 (L2Z43_16165) pilT 3405867..3406904 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Z43_RS16170 (L2Z43_16170) - 3407031..3407723 (+) 693 WP_001108520.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2Z43_RS16175 (L2Z43_16175) - 3407924..3411520 (-) 3597 WP_000698801.1 AAA family ATPase -
  L2Z43_RS16180 (L2Z43_16180) - 3411530..3412798 (-) 1269 WP_001984248.1 exonuclease SbcCD subunit D -
  L2Z43_RS16185 (L2Z43_16185) - 3412963..3413436 (+) 474 WP_002134289.1 OsmC family protein -
  L2Z43_RS16190 (L2Z43_16190) - 3413510..3413887 (-) 378 WP_001059437.1 VOC family protein -
  L2Z43_RS16195 (L2Z43_16195) - 3414267..3414719 (+) 453 WP_000164154.1 ABZJ_00895 family protein -
  L2Z43_RS16200 (L2Z43_16200) - 3414808..3415713 (+) 906 WP_001039359.1 TIGR01777 family oxidoreductase -
  L2Z43_RS16205 (L2Z43_16205) - 3415705..3416820 (-) 1116 WP_000546700.1 FAD-dependent oxidoreductase -
  L2Z43_RS16210 (L2Z43_16210) hemB 3416846..3417859 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  L2Z43_RS16215 (L2Z43_16215) - 3418002..3418499 (+) 498 WP_002019577.1 thioesterase family protein -
  L2Z43_RS16220 (L2Z43_16220) - 3418769..3419920 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  L2Z43_RS16225 (L2Z43_16225) - 3419927..3421450 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  L2Z43_RS16230 (L2Z43_16230) ggt 3421754..3423739 (+) 1986 WP_001042928.1 gamma-glutamyltransferase -
  L2Z43_RS16240 (L2Z43_16240) - 3424440..3425669 (+) 1230 WP_002134292.1 tyrosine-type recombinase/integrase -
  L2Z43_RS16245 (L2Z43_16245) - 3425819..3426445 (+) 627 WP_000151565.1 hypothetical protein -
  L2Z43_RS16250 (L2Z43_16250) - 3426573..3426788 (+) 216 WP_000153154.1 hypothetical protein -
  L2Z43_RS16255 (L2Z43_16255) - 3426791..3427000 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  L2Z43_RS16260 (L2Z43_16260) - 3426997..3427551 (+) 555 WP_000991775.1 BRO family protein -
  L2Z43_RS16265 (L2Z43_16265) - 3427544..3427912 (+) 369 WP_001046480.1 hypothetical protein -
  L2Z43_RS16270 (L2Z43_16270) - 3427922..3428233 (+) 312 WP_000787400.1 hypothetical protein -
  L2Z43_RS16275 (L2Z43_16275) - 3428348..3428626 (+) 279 WP_001064706.1 hypothetical protein -
  L2Z43_RS16280 (L2Z43_16280) - 3428638..3431103 (+) 2466 WP_001022433.1 DUF927 domain-containing protein -
  L2Z43_RS16285 (L2Z43_16285) - 3431427..3431630 (+) 204 WP_001984265.1 single-stranded DNA-binding protein -
  L2Z43_RS16290 (L2Z43_16290) - 3431767..3433209 (+) 1443 WP_000003532.1 hypothetical protein -
  L2Z43_RS16295 (L2Z43_16295) - 3433552..3434226 (+) 675 WP_000560650.1 DUF3800 domain-containing protein -
  L2Z43_RS16300 (L2Z43_16300) - 3434226..3434414 (+) 189 WP_000642900.1 hypothetical protein -
  L2Z43_RS16305 (L2Z43_16305) - 3434559..3435134 (+) 576 WP_000067109.1 hypothetical protein -
  L2Z43_RS19130 - 3435606..3435768 (+) 163 Protein_3164 single-stranded DNA-binding protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41905.11 Da        Isoelectric Point: 6.6944

>NTDB_id=649346 L2Z43_RS16160 WP_000347036.1 3404721..3405839(-) (pilU) [Acinetobacter baumannii strain ATCC19606-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=649346 L2Z43_RS16160 WP_000347036.1 3404721..3405839(-) (pilU) [Acinetobacter baumannii strain ATCC19606-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PAD6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.293

98.925

0.685
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

93.011

0.392