Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y54_RS06955 Genome accession   NZ_CP091244
Coordinates   1370097..1371275 (-) Length   392 a.a.
NCBI ID   WP_236501093.1    Uniprot ID   -
Organism   Thiothrix winogradskyi strain CT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1365097..1376275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y54_RS06940 (L2Y54_06940) yciA 1367120..1367512 (+) 393 WP_236501088.1 acyl-CoA thioester hydrolase YciA -
  L2Y54_RS06945 (L2Y54_06945) - 1367516..1368247 (-) 732 WP_236501089.1 M48 family metallopeptidase -
  L2Y54_RS06950 (L2Y54_06950) argS 1368348..1370096 (+) 1749 WP_236501091.1 arginine--tRNA ligase -
  L2Y54_RS06955 (L2Y54_06955) pilU 1370097..1371275 (-) 1179 WP_236501093.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y54_RS06960 (L2Y54_06960) - 1371328..1372026 (+) 699 WP_236501094.1 pseudouridine synthase -
  L2Y54_RS06965 (L2Y54_06965) - 1372016..1375456 (-) 3441 WP_236501095.1 InlB B-repeat-containing protein -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43784.68 Da        Isoelectric Point: 7.1149

>NTDB_id=648790 L2Y54_RS06955 WP_236501093.1 1370097..1371275(-) (pilU) [Thiothrix winogradskyi strain CT3]
MKLKDYLRILAHYDGSDLYLTADLEPKAKFQGKLKAVDKVIMTPDMLKAMAYELMNPEQQQQFEKKPEMNLAISEEGIGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTVDGKRVAAIEILLGTPMVRDLIMKGDVHAIKETMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLNKPRPTAAAPAPEAKPKAADEDFMAKLSLQPKPEAELT

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=648790 L2Y54_RS06955 WP_236501093.1 1370097..1371275(-) (pilU) [Thiothrix winogradskyi strain CT3]
ATGAAACTCAAAGACTATCTACGCATCCTCGCCCATTACGACGGCTCTGACCTGTATTTAACCGCTGATCTCGAACCCAA
AGCCAAATTTCAGGGCAAGCTCAAAGCGGTCGATAAAGTCATCATGACCCCCGACATGCTCAAAGCAATGGCTTACGAGC
TGATGAACCCTGAACAGCAGCAACAGTTTGAAAAGAAACCCGAAATGAACCTCGCCATCAGCGAAGAAGGTATCGGGCGT
TTCCGCGTCAATATCTTCAAGCAGCGCCACAAAATCGCGATGGTGATCCGCAATATCAAAACCGACATACCCAACGCCGA
CAAACTCGGTCTACCGCAAGTTTTGAAAGACGTGATCATGGAAAAACGCGGGCTAATCCTATTCGTTGGCGGCACAGGTT
CAGGCAAATCCACCTCACTCGCCGCGCTGATCGACCACCGCAACGCTAGTGCGGACGGGCACATCATCACCATCGAAGAC
CCGGTGGAATACGTGCATCCACACAAAAAATGCATCATTAACCAGCGCGAAGTCGGCGTAGACACCGACAGCTACGAAGA
CGCACTCAAAAACACCCTGCGCCAAGCTCCCGACGTGATTTTAATCGGCGAAATCCGCGCCCAAGAAACGATGGAACACG
CCCTCGCCTTCGCCGAAACCGGGCATCTGTGCCTCTCCACCTTGCACGCCAATAACGCCAACCAAGCCCTCGACCGCATT
ATCAACTTCTTCCCCGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATTCCCACGGTGGATGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACGCCAATGGTGCGCGACCTGATCA
TGAAAGGCGACGTGCACGCCATCAAAGAAACGATGGAAAAGTCTGAAGAACAGGGGATGCAAACCTTCGACAGCCACCTC
TACAAGCTGTATTTGGCGGGGCAAATTTCACTGGCGGAAACCTTGCGCAATGCTGATTCGCCTAGCAACTTGAAGCTGAA
GATCAACCTGTCAGGGAATTTGAATAAGCCACGTCCGACGGCTGCCGCACCTGCGCCAGAGGCAAAGCCCAAGGCGGCAG
ATGAAGACTTCATGGCGAAATTGTCGTTACAGCCAAAACCAGAAGCAGAGCTGACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.769

92.857

0.518
  pilU Acinetobacter baylyi ADP1

55.153

91.582

0.505
  pilU Vibrio cholerae strain A1552

49.57

89.031

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.896

93.367

0.401