Detailed information NTG0648068

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LQS91_RS05210
• Genome accession: NZ_CP091172
• Coordinates: 1019616..1020734 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain MRSN57
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1014616..1025734

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LQS91_RS05180 (LQS91_05180)	-	1015445..1016293 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
LQS91_RS05185 (LQS91_05185)	-	1016546..1017001 (+)	456	WP_000782976.1	bacteriohemerythrin	-
LQS91_RS05190 (LQS91_05190)	-	1017154..1018221 (+)	1068	WP_000092403.1	hypothetical protein	-
LQS91_RS05195 (LQS91_05195)	-	1018221..1018544 (+)	324	WP_000442585.1	RnfH family protein	-
LQS91_RS05200 (LQS91_05200)	-	1018599..1018997 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LQS91_RS05205 (LQS91_05205)	fur	1019109..1019546 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LQS91_RS05210 (LQS91_05210)	pilU	1019616..1020734 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LQS91_RS05215 (LQS91_05215)	pilT	1020762..1021799 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
LQS91_RS05220 (LQS91_05220)	-	1021926..1022618 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=648068 LQS91_RS05210 WP_000347036.1 1019616..1020734(-) (pilU) [Acinetobacter baumannii strain MRSN57]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=648068 LQS91_RS05210 WP_000347036.1 1019616..1020734(-) (pilU) [Acinetobacter baumannii strain MRSN57]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392