Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   L2Y01_RS02390 Genome accession   NZ_CP091147
Coordinates   488893..489957 (-) Length   354 a.a.
NCBI ID   WP_003378852.1    Uniprot ID   A0A2G9KZJ9
Organism   Pseudomonas avellanae strain NCPPB 4222     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483893..494957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y01_RS02365 (L2Y01_02365) aroK 484447..484965 (-) 519 WP_003378847.1 shikimate kinase AroK -
  L2Y01_RS02370 (L2Y01_02370) pilQ 484970..487075 (-) 2106 WP_005621204.1 type IV pilus secretin PilQ Machinery gene
  L2Y01_RS02375 (L2Y01_02375) pilP 487123..487650 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  L2Y01_RS02380 (L2Y01_02380) pilO 487647..488270 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  L2Y01_RS02385 (L2Y01_02385) - 488267..488893 (-) 627 WP_005621206.1 PilN domain-containing protein -
  L2Y01_RS02390 (L2Y01_02390) pilM 488893..489957 (-) 1065 WP_003378852.1 pilus assembly protein PilM Machinery gene
  L2Y01_RS02395 (L2Y01_02395) - 490164..492608 (+) 2445 WP_005621209.1 penicillin-binding protein 1A -
  L2Y01_RS02400 (L2Y01_02400) - 492951..494219 (-) 1269 WP_003378855.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38103.62 Da        Isoelectric Point: 4.7008

>NTDB_id=648027 L2Y01_RS02390 WP_003378852.1 488893..489957(-) (pilM) [Pseudomonas avellanae strain NCPPB 4222]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=648027 L2Y01_RS02390 WP_003378852.1 488893..489957(-) (pilM) [Pseudomonas avellanae strain NCPPB 4222]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G9KZJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469