Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1F30_RS10225 Genome accession   NZ_CP090959
Coordinates   2305213..2306406 (+) Length   397 a.a.
NCBI ID   WP_253355945.1    Uniprot ID   -
Organism   Simiduia sp. 21SJ11W-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2300213..2311406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F30_RS10205 (L1F30_10205) - 2300775..2301545 (+) 771 WP_253355937.1 sulfite exporter TauE/SafE family protein -
  L1F30_RS10210 (L1F30_10210) - 2301566..2302522 (-) 957 WP_253355939.1 peptidylprolyl isomerase -
  L1F30_RS10215 (L1F30_10215) - 2302699..2303313 (+) 615 WP_253355941.1 TetR/AcrR family transcriptional regulator -
  L1F30_RS10220 (L1F30_10220) - 2303347..2305014 (+) 1668 WP_253355943.1 acyl-CoA dehydrogenase family protein -
  L1F30_RS10225 (L1F30_10225) pilU 2305213..2306406 (+) 1194 WP_253355945.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1F30_RS10230 (L1F30_10230) - 2306504..2306731 (-) 228 WP_253355947.1 hypothetical protein -
  L1F30_RS10235 (L1F30_10235) - 2306869..2308392 (-) 1524 WP_253355948.1 tryptophan halogenase family protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 43918.24 Da        Isoelectric Point: 5.7232

>NTDB_id=646686 L1F30_RS10225 WP_253355945.1 2305213..2306406(+) (pilU) [Simiduia sp. 21SJ11W-1]
MATPIDNLLNILAKKDGSDLYLSTGAPPCAKFQGALKPLSQTPYSSGEIEGIANEIMDNEQRSIFNEELEMNLAISIAGV
GRFRINIFKQRNEVSIVARNIKAEIPKFADLKLPEVLKELILTKRGLILFVGATGSGKSTSLAALIDHRNSTSGGHIITI
EDPVEYVHKHKKSIINQREVGVDTRSFRNALKNTLRQAPDVILIGEIRDRETMEHALEFADTGHLAISTLHANNANQALE
RIINFFPEERRPQLLLSLSQNLRAFVSQRLIPTIDGKRCAAVEILIGTKTIQEMILKARLEEIKEIMEKSENLGMQTFDG
ALFHLYKEGRISQEEALANADSPNNLRLRIKLDSRKAGTPEPATPAPTPQAEAPAQAMSFGELSLEKMDDEEGEEAP

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=646686 L1F30_RS10225 WP_253355945.1 2305213..2306406(+) (pilU) [Simiduia sp. 21SJ11W-1]
ATGGCCACGCCGATCGACAATTTACTGAACATTCTCGCCAAAAAAGACGGGTCAGACCTCTACCTGAGCACCGGCGCACC
ACCCTGCGCGAAGTTTCAGGGCGCACTTAAGCCGCTGTCGCAAACGCCCTACAGCTCTGGTGAGATAGAGGGCATCGCCA
ACGAAATAATGGATAATGAGCAGCGCTCTATTTTTAATGAAGAGCTGGAAATGAATCTGGCCATATCCATCGCAGGCGTC
GGCCGGTTTCGCATTAACATTTTCAAGCAGCGCAACGAAGTCTCGATTGTGGCGCGCAACATCAAAGCCGAAATTCCAAA
ATTTGCAGACCTCAAACTGCCTGAAGTATTAAAAGAGCTGATTCTCACCAAACGCGGGCTCATTTTGTTTGTGGGCGCCA
CAGGCTCCGGCAAGTCCACCTCCCTTGCAGCCTTGATTGATCACCGCAACAGCACCTCTGGCGGGCACATCATCACCATT
GAAGACCCGGTTGAATATGTGCACAAACACAAGAAGAGCATCATCAACCAACGCGAAGTGGGGGTAGATACCCGCAGTTT
CAGAAACGCCCTGAAAAACACGCTGCGCCAGGCGCCCGATGTGATTTTAATTGGCGAGATTCGCGACCGCGAAACCATGG
AGCACGCACTGGAATTTGCCGACACCGGCCATTTGGCAATTTCTACGCTGCATGCCAATAACGCCAATCAGGCCTTAGAA
AGAATTATCAACTTCTTCCCGGAAGAGCGGCGCCCTCAACTGTTACTTTCGCTTTCGCAAAACTTGCGTGCCTTTGTGTC
TCAACGCCTAATTCCCACCATAGACGGCAAGCGCTGCGCGGCGGTAGAGATTTTAATAGGCACCAAAACCATTCAGGAAA
TGATTCTAAAAGCGCGCCTGGAAGAAATTAAAGAGATTATGGAGAAATCCGAAAACCTCGGCATGCAAACCTTCGATGGC
GCCCTCTTTCACCTCTATAAAGAAGGCCGCATCAGCCAGGAAGAAGCCCTCGCCAATGCCGACTCCCCCAACAACTTGCG
CCTGCGCATTAAACTCGACAGCCGCAAGGCAGGCACACCTGAGCCCGCAACACCCGCCCCCACGCCGCAAGCAGAAGCGC
CCGCGCAGGCCATGTCTTTCGGTGAGCTATCGCTGGAAAAAATGGATGATGAAGAAGGTGAAGAGGCACCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.775

89.421

0.499
  pilU Acinetobacter baylyi ADP1

54.19

90.176

0.489
  pilU Vibrio cholerae strain A1552

47.701

87.657

0.418
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

92.191

0.363