Detailed information NTG0644212

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L0025_RS13870
• Genome accession: NZ_CP090648
• Coordinates: 2975036..2976184 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA1616
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2970036..2981184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L0025_RS13845 (L0025_13845)	-	2971187..2971483 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
L0025_RS13850 (L0025_13850)	-	2971480..2972073 (-)	594	WP_003084544.1	YggT family protein	-
L0025_RS13855 (L0025_13855)	proC	2972084..2972905 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
L0025_RS13860 (L0025_13860)	-	2972917..2973609 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
L0025_RS13865 (L0025_13865)	pilT	2973824..2974858 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
L0025_RS13870 (L0025_13870)	pilU	2975036..2976184 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
L0025_RS13875 (L0025_13875)	czcD	2976192..2977091 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
L0025_RS13880 (L0025_13880)	-	2977245..2977649 (+)	405	WP_003084560.1	NINE protein	-
L0025_RS13885 (L0025_13885)	-	2977892..2979265 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
L0025_RS13890 (L0025_13890)	-	2979262..2980446 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=644212 L0025_RS13870 WP_003100959.1 2975036..2976184(+) (pilU) [Pseudomonas aeruginosa strain PA1616]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=644212 L0025_RS13870 WP_003100959.1 2975036..2976184(+) (pilU) [Pseudomonas aeruginosa strain PA1616]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382