Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LPY95_RS07120 Genome accession   NZ_CP090564
Coordinates   1699047..1700177 (+) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas citri pv. malvacearum strain CFBP 2012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1694047..1705177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPY95_RS07090 (LPY95_07100) - 1694158..1694799 (-) 642 WP_033479080.1 M23 family metallopeptidase -
  LPY95_RS07095 (LPY95_07105) - 1694906..1695331 (-) 426 WP_003489904.1 HU family DNA-binding protein -
  LPY95_RS07100 (LPY95_07110) - 1695472..1695864 (-) 393 WP_125825487.1 hypothetical protein -
  LPY95_RS07105 (LPY95_07115) proC 1695885..1696739 (-) 855 WP_269340260.1 pyrroline-5-carboxylate reductase -
  LPY95_RS07110 (LPY95_07120) - 1696783..1697475 (-) 693 WP_269340261.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPY95_RS07115 (LPY95_07125) pilT 1697887..1698924 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  LPY95_RS07120 (LPY95_07130) pilU 1699047..1700177 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPY95_RS07125 (LPY95_07135) - 1700671..1701345 (+) 675 WP_033479081.1 YitT family protein -
  LPY95_RS07130 (LPY95_07140) - 1701520..1702446 (-) 927 WP_172107359.1 DUF72 domain-containing protein -
  LPY95_RS07135 (LPY95_07145) - 1702454..1703026 (-) 573 WP_005919206.1 DNA-3-methyladenine glycosylase I -
  LPY95_RS07140 (LPY95_07150) - 1703315..1703881 (+) 567 WP_005913286.1 YqgE/AlgH family protein -
  LPY95_RS07145 (LPY95_07155) ruvX 1703874..1704341 (+) 468 WP_003489932.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=643502 LPY95_RS07120 WP_003489921.1 1699047..1700177(+) (pilU) [Xanthomonas citri pv. malvacearum strain CFBP 2012]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=643502 LPY95_RS07120 WP_003489921.1 1699047..1700177(+) (pilU) [Xanthomonas citri pv. malvacearum strain CFBP 2012]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGATCTATCGCTGAATCTCAAGGGTGTGGT
GGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGTCTGGATGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364