Detailed information NTG0064322

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SCV20265_RS02030
• Genome accession: NC_023149
• Coordinates: 437631..438779 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa SCV20265
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 432631..443779

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SCV20265_RS02005	-	433782..434078 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
SCV20265_RS02010 (SCV20265_0409)	-	434075..434668 (-)	594	WP_003084544.1	YggT family protein	-
SCV20265_RS02015 (SCV20265_0410)	proC	434679..435500 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
SCV20265_RS02020 (SCV20265_0411)	-	435512..436204 (-)	693	WP_023122937.1	YggS family pyridoxal phosphate-dependent enzyme	-
SCV20265_RS02025 (SCV20265_0412)	pilT	436419..437453 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
SCV20265_RS02030 (SCV20265_0413)	pilU	437631..438779 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
SCV20265_RS02035 (SCV20265_0414)	czcD	438787..439686 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
SCV20265_RS02040 (SCV20265_0415)	-	439840..440244 (+)	405	WP_003084560.1	NINE protein	-
SCV20265_RS02045 (SCV20265_0416)	-	440487..441860 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
SCV20265_RS02050 (SCV20265_0417)	-	441857..443041 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=64322 SCV20265_RS02030 WP_003110016.1 437631..438779(+) (pilU) [Pseudomonas aeruginosa SCV20265]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=64322 SCV20265_RS02030 WP_003110016.1 437631..438779(+) (pilU) [Pseudomonas aeruginosa SCV20265]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382