Detailed information NTG0643214

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA22_RS02015
• Genome accession: NZ_CP107275
• Coordinates: 433898..435046 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 428898..440046

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA22_RS01990	-	430049..430345 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA22_RS01995 (PALA22_00400)	-	430342..430935 (-)	594	WP_003084544.1	YggT family protein	-
PALA22_RS02000 (PALA22_00401)	proC	430946..431767 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PALA22_RS02005 (PALA22_00402)	-	431779..432471 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA22_RS02010 (PALA22_00403)	pilT	432686..433720 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA22_RS02015 (PALA22_00404)	pilU	433898..435046 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA22_RS02020 (PALA22_00405)	czcD	435054..435953 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA22_RS02025 (PALA22_00406)	-	436108..436512 (+)	405	WP_003084560.1	NINE protein	-
PALA22_RS02030 (PALA22_00407)	-	436755..438128 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA22_RS02035 (PALA22_00408)	-	438125..439309 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=643214 PALA22_RS02015 WP_003100959.1 433898..435046(+) (pilU) [Pseudomonas aeruginosa strain PALA22]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=643214 PALA22_RS02015 WP_003100959.1 433898..435046(+) (pilU) [Pseudomonas aeruginosa strain PALA22]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382