Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LY388_RS06785 Genome accession   NZ_CP090387
Coordinates   278540..279130 (+) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae strain GXFL1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273540..284130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY388_RS06765 (LY388_06765) tnpA 273810..274248 (+) 439 Protein_238 IS200/IS605-like element IS1004 family transposase -
  LY388_RS06770 (LY388_06770) - 274337..276814 (-) 2478 WP_032470274.1 penicillin-binding protein 1A -
  LY388_RS06775 (LY388_06775) pilM 276957..277973 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  LY388_RS06780 (LY388_06780) pilN 277963..278547 (+) 585 WP_185840471.1 PilN domain-containing protein Machinery gene
  LY388_RS06785 (LY388_06785) pilO 278540..279130 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  LY388_RS06790 (LY388_06790) pilP 279120..279638 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  LY388_RS06795 (LY388_06795) pilQ 279664..281379 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  LY388_RS06800 (LY388_06800) aroK 281566..282090 (+) 525 WP_000818616.1 shikimate kinase AroK -
  LY388_RS06805 (LY388_06805) aroB 282118..283203 (+) 1086 WP_162810142.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=641913 LY388_RS06785 WP_000157690.1 278540..279130(+) (pilO) [Vibrio cholerae strain GXFL1-4]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=641913 LY388_RS06785 WP_000157690.1 278540..279130(+) (pilO) [Vibrio cholerae strain GXFL1-4]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATCAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCCCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGTCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694