Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LY388_RS06775 Genome accession   NZ_CP090387
Coordinates   276957..277973 (+) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae strain GXFL1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271957..282973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY388_RS06760 (LY388_06760) oxyR 272745..273638 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  LY388_RS06765 (LY388_06765) tnpA 273810..274248 (+) 439 Protein_238 IS200/IS605-like element IS1004 family transposase -
  LY388_RS06770 (LY388_06770) - 274337..276814 (-) 2478 WP_032470274.1 penicillin-binding protein 1A -
  LY388_RS06775 (LY388_06775) pilM 276957..277973 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  LY388_RS06780 (LY388_06780) pilN 277963..278547 (+) 585 WP_185840471.1 PilN domain-containing protein Machinery gene
  LY388_RS06785 (LY388_06785) pilO 278540..279130 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  LY388_RS06790 (LY388_06790) pilP 279120..279638 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  LY388_RS06795 (LY388_06795) pilQ 279664..281379 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  LY388_RS06800 (LY388_06800) aroK 281566..282090 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=641911 LY388_RS06775 WP_000520444.1 276957..277973(+) (pilM) [Vibrio cholerae strain GXFL1-4]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=641911 LY388_RS06775 WP_000520444.1 276957..277973(+) (pilM) [Vibrio cholerae strain GXFL1-4]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCT
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCTCGCAGCAGTACGA
CGACCTTCCAAGTGTATGCAACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGTGAAG
TACTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485