Detailed information NTG0640358

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA20_RS05380
• Genome accession: NZ_CP107064
• Coordinates: 1123439..1124587 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1118439..1129587

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA20_RS05360 (PALA20_01062)	-	1119176..1120360 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
PALA20_RS05365 (PALA20_01063)	-	1120357..1121730 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PALA20_RS05370 (PALA20_01064)	-	1121973..1122377 (-)	405	WP_003084560.1	NINE protein	-
PALA20_RS05375 (PALA20_01065)	czcD	1122532..1123431 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
PALA20_RS05380 (PALA20_01066)	pilU	1123439..1124587 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA20_RS05385 (PALA20_01067)	pilT	1124765..1125799 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA20_RS05390 (PALA20_01068)	-	1126014..1126706 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA20_RS05395 (PALA20_01069)	proC	1126718..1127539 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA20_RS05400 (PALA20_01070)	-	1127550..1128143 (+)	594	WP_003084544.1	YggT family protein	-
PALA20_RS05405	-	1128140..1128436 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=640358 PALA20_RS05380 WP_003100959.1 1123439..1124587(-) (pilU) [Pseudomonas aeruginosa strain PALA20]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=640358 PALA20_RS05380 WP_003100959.1 1123439..1124587(-) (pilU) [Pseudomonas aeruginosa strain PALA20]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382