Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LWM38_RS01225 Genome accession   NZ_CP090019
Coordinates   274881..275492 (+) Length   203 a.a.
NCBI ID   WP_060468742.1    Uniprot ID   A0A120DG30
Organism   Vibrio kanaloae strain KH-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269881..280492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LWM38_RS01210 (LWM38_01210) - 270567..273128 (-) 2562 WP_233894526.1 penicillin-binding protein 1A -
  LWM38_RS01215 (LWM38_01215) pilM 273280..274305 (+) 1026 WP_017055836.1 type IV pilus assembly protein PilM Machinery gene
  LWM38_RS01220 (LWM38_01220) pilN 274289..274888 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  LWM38_RS01225 (LWM38_01225) pilO 274881..275492 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  LWM38_RS01230 (LWM38_01230) pilP 275479..275997 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  LWM38_RS01235 (LWM38_01235) pilQ 276057..277748 (+) 1692 WP_233894528.1 type IV pilus secretin PilQ Machinery gene
  LWM38_RS01240 (LWM38_01240) aroK 277925..278443 (+) 519 WP_017061125.1 shikimate kinase AroK -
  LWM38_RS01245 (LWM38_01245) aroB 278469..279557 (+) 1089 WP_077680945.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23477.94 Da        Isoelectric Point: 4.7445

>NTDB_id=640235 LWM38_RS01225 WP_060468742.1 274881..275492(+) (pilO) [Vibrio kanaloae strain KH-4]
MVSFQNRVSLQDLDVDEITEWPLLPQLLVIFALMILIQGVGTWLYIAPLDDELQQMKQQEQTLKTTLRIKANKAAVLPKL
QSQLDDLTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHAIGDFSAAIA
KLPRIISFNDVSWQRVSQESSTLHFRVRAYTYQFKPELSDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=640235 LWM38_RS01225 WP_060468742.1 274881..275492(+) (pilO) [Vibrio kanaloae strain KH-4]
ATGGTTAGTTTTCAAAATCGGGTGAGCTTGCAAGATCTCGATGTCGATGAGATCACCGAATGGCCTTTATTGCCTCAGCT
ATTGGTCATTTTTGCCTTGATGATACTTATCCAAGGTGTGGGTACTTGGTTGTATATTGCTCCTTTGGACGATGAACTTC
AGCAGATGAAACAGCAAGAGCAGACGCTTAAAACAACTTTGCGGATTAAAGCCAATAAGGCGGCGGTTTTGCCTAAGCTC
CAGAGTCAGTTGGATGATTTAACCAGTCGTTATGATTATCTATTAGAGCAGCTACCAGTACAAAAAGAGTTGGCAAGTAT
GTTGGCCTCAGTGAATGAGCTTGGGTTGGATAATAAGCTGACGTTTACGCGTATTGATTGGGGACAGAAGCAAAACAAGG
AGTTTCTCTATCGTTTACCTCTCAATATAGAGCTAACAGGGGACTATCACGCGATTGGAGACTTCTCTGCAGCCATCGCC
AAACTCCCACGAATCATTAGTTTTAATGATGTGAGTTGGCAGCGCGTCAGCCAAGAAAGTAGCACGCTCCATTTCAGGGT
GCGAGCGTATACCTACCAATTTAAGCCGGAGTTGAGTGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A120DG30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

73.711

95.567

0.704
  pilO Vibrio campbellii strain DS40M4

70.37

93.103

0.655