Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LWM38_RS01235 Genome accession   NZ_CP090019
Coordinates   276057..277748 (+) Length   563 a.a.
NCBI ID   WP_233894528.1    Uniprot ID   -
Organism   Vibrio kanaloae strain KH-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271057..282748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LWM38_RS01215 (LWM38_01215) pilM 273280..274305 (+) 1026 WP_017055836.1 type IV pilus assembly protein PilM Machinery gene
  LWM38_RS01220 (LWM38_01220) pilN 274289..274888 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  LWM38_RS01225 (LWM38_01225) pilO 274881..275492 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  LWM38_RS01230 (LWM38_01230) pilP 275479..275997 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  LWM38_RS01235 (LWM38_01235) pilQ 276057..277748 (+) 1692 WP_233894528.1 type IV pilus secretin PilQ Machinery gene
  LWM38_RS01240 (LWM38_01240) aroK 277925..278443 (+) 519 WP_017061125.1 shikimate kinase AroK -
  LWM38_RS01245 (LWM38_01245) aroB 278469..279557 (+) 1089 WP_077680945.1 3-dehydroquinate synthase -
  LWM38_RS01250 (LWM38_01250) - 279637..281142 (+) 1506 WP_233894530.1 SPOR domain-containing protein -
  LWM38_RS01255 (LWM38_01255) - 281212..282072 (+) 861 WP_046224649.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61359.95 Da        Isoelectric Point: 4.4325

>NTDB_id=640237 LWM38_RS01235 WP_233894528.1 276057..277748(+) (pilQ) [Vibrio kanaloae strain KH-4]
MNKGLQGLIVSVMLIFSVLSHAEDLPNKLENIDFRVNKNKDAVLIIELATSTVVVDVQRAQEGLSIELINTHVDDNKLQL
LDVKDFATLVEGVEVYKETPSTRLLATISKDYQYEYNLKGRFIEVVISKPAIEEAVTEKSILEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREQQALEKSRLEEELG
ELKSEIIKIHFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRSIIESLDVPVKQVQIEARIVTV
TEGNLDELGVRWGASSTNGSFTVGGSIEGNYPSITPYDSNGGNSVIDEYLNVNLGATSSNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=640237 LWM38_RS01235 WP_233894528.1 276057..277748(+) (pilQ) [Vibrio kanaloae strain KH-4]
ATGAACAAAGGGTTACAAGGCTTGATTGTGTCTGTGATGTTGATCTTTAGTGTGCTCAGTCACGCTGAGGACTTACCTAA
TAAGTTAGAAAATATTGATTTTAGGGTGAACAAAAATAAAGACGCTGTCCTAATTATCGAGCTCGCAACCAGCACGGTTG
TGGTGGATGTTCAACGTGCACAAGAGGGGCTAAGCATAGAGCTGATCAATACTCATGTTGATGACAACAAACTCCAGCTT
CTTGATGTGAAAGATTTCGCTACCTTGGTTGAAGGCGTGGAAGTGTATAAAGAGACCCCAAGCACTCGGTTGTTAGCGAC
AATCAGCAAGGATTACCAATACGAATACAATCTCAAAGGCCGTTTTATTGAGGTGGTGATCAGTAAACCTGCTATAGAAG
AAGCGGTAACAGAGAAAAGTATACTCGAGAAAGAGGGTAAGCTGATATCGATTAACTTCCAAGATATTCCAGTGCGAAAT
GTCTTGCAGCTTATCGCTGACTATAACGACTTTAACTTAGTCGTGTCTGATTCAGTAACCGGAAACCTAACTTTACGTTT
AGATGGTGTGCCCTGGCAGCAAGTTCTGGATATCATATTACAGGTCAAAGGGTTAGATAAGCGGGTAGATGGCAATGTCA
TTTTGGTAGCGCCTAAAGATGAACTCGACCTTCGAGAGCAGCAAGCGTTAGAGAAGTCGCGTTTAGAAGAGGAATTGGGT
GAGCTTAAGTCGGAAATTATTAAGATTCACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGAGGCGAGGGGGCGGT
GAGTATGTTGTCTGATCGGGGCTCGATTACTATTGATGAGCGCACTAATTCATTGCTTATTCGTGAACTGGAGGAAAATA
TTGCGGTGATACGAAGCATTATTGAATCTTTAGATGTCCCTGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTGACTGTG
ACTGAGGGGAACCTTGATGAGCTAGGAGTGCGTTGGGGAGCTTCTTCAACAAACGGAAGCTTCACGGTTGGTGGTTCTAT
CGAAGGCAACTACCCGTCTATTACACCTTACGATAGTAATGGAGGGAATAGCGTCATTGATGAGTACCTCAACGTTAATT
TAGGTGCGACATCTTCAAATGCTTCGAGTATCGCATTTCAGGTTGCTAAACTGGGCTCTGATACTCTGCTCGACCTTGAG
TTATCAGCGTTACAACAAGAGTCAAAAGCTGAAATTATTTCGAGTCCACGTTTGATTACTACCAATAAAAAGCCCGCTTA
TATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCATCTTCAAGTGGTGCAACGTCAGTCGCATTTAAGAAAGCGGTAT
TGAGCCTTAAGGTGACGCCGCAGATCACGCCAGACAATCGTTTGGTGTTGGATTTGAGCGTAACTCAAGATAGGCCCGGA
CAGGTGGTGAAAACCGGAACTGGAGAGGCTGTTGCAATTGATACCCAGCGTATTGGCACGCAAGTGCTTGTTAATAATGG
TGAAACAGTTGTTCTTGGTGGGATATTTCAGCACAGTGTGAGTAGCACCGTCGATAAAGTTCCTCTATTGGGAGACCTGC
CTGTATTAGGGGCTTTGTTCCGCAGAAGCTACGAAAATGTAGGGAAAAGTGAGTTGTTGATCTTTGTTACACCGAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

78.938

96.98

0.766
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.938

96.98

0.766
  pilQ Vibrio campbellii strain DS40M4

79.374

96.448

0.766
  pilQ Legionella pneumophila strain Lp02

42.125

96.98

0.409
  pilQ Legionella pneumophila strain ERS1305867

42.125

96.98

0.409
  pilQ Pseudomonas aeruginosa PAK

40.827

98.757

0.403
  comQ Acinetobacter baylyi ADP1

39.107

99.467

0.389
  pilQ Acinetobacter baumannii D1279779

37.37

100

0.384
  pilQ Acinetobacter baumannii strain A118

37.37

100

0.384