Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LWM38_RS01215 Genome accession   NZ_CP090019
Coordinates   273280..274305 (+) Length   341 a.a.
NCBI ID   WP_017055836.1    Uniprot ID   A0A4U1X938
Organism   Vibrio kanaloae strain KH-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268280..279305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LWM38_RS01200 (LWM38_01200) - 268630..269358 (-) 729 WP_017055839.1 glutathione peroxidase -
  LWM38_RS01205 (LWM38_01205) oxyR 269578..270465 (+) 888 WP_137008133.1 DNA-binding transcriptional regulator OxyR -
  LWM38_RS01210 (LWM38_01210) - 270567..273128 (-) 2562 WP_233894526.1 penicillin-binding protein 1A -
  LWM38_RS01215 (LWM38_01215) pilM 273280..274305 (+) 1026 WP_017055836.1 type IV pilus assembly protein PilM Machinery gene
  LWM38_RS01220 (LWM38_01220) pilN 274289..274888 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  LWM38_RS01225 (LWM38_01225) pilO 274881..275492 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  LWM38_RS01230 (LWM38_01230) pilP 275479..275997 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  LWM38_RS01235 (LWM38_01235) pilQ 276057..277748 (+) 1692 WP_233894528.1 type IV pilus secretin PilQ Machinery gene
  LWM38_RS01240 (LWM38_01240) aroK 277925..278443 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37975.53 Da        Isoelectric Point: 7.2208

>NTDB_id=640233 LWM38_RS01215 WP_017055836.1 273280..274305(+) (pilM) [Vibrio kanaloae strain KH-4]
MGSSLITGIDINHHSIKAVVLKPVGELYAIVGYKELPISDDIFTANHTLEYQKTVKKLKELKKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRANALSKAGFKP
IVVDTQAHGLLNIWQLASYLSPEKKNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQELLGTGSPSDMEGVFGTTQDTH
RFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASIPMVVEELERHFQMSCESLNPMSLFENKVAQKRRSSLNCQHFG
IAAGMAMRGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=640233 LWM38_RS01215 WP_017055836.1 273280..274305(+) (pilM) [Vibrio kanaloae strain KH-4]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGTATCAAAGCTGTGGTACTAAAACCCGTTGGGGAGTT
GTATGCCATCGTGGGATACAAAGAGCTGCCTATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAAGAAAGGACTGCCTTTTGGAAGTCGTAACGTCGCTATTTCAGTACCAGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGATAGAGAGAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAAGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGGTTTGGTAAAGGATCGACAA
GTAGTTATCAGGTGTACGCGACTCGCAAGGAAGTTGTGGATAGTCGAGCCAATGCATTAAGCAAAGCCGGGTTTAAACCT
ATCGTCGTTGATACCCAAGCACACGGACTTCTCAATATTTGGCAATTGGCTTCGTATTTGTCCCCAGAAAAGAAGAATTG
GCTGCTGGTGGACGTTGGAATCGACCAAACCTCGTTAGGGGTTATCCCGCAGAATTCTGCGCCTTTTTATAAAGACATCG
CCTTTGGTACTCAAGAGCTGTTAGGCACAGGCAGTCCAAGTGATATGGAAGGCGTGTTCGGCACCACTCAGGATACACAT
CGGTTTATTGTTAATCTTATTGAAAAGCTCAAGCGTCAACTGCAACTCTATTCGTCGGTAAATGCGCTTCAACCTATCTC
AGGGATTTGGCTAATGGGCGAAGGAGCGAGCATACCAATGGTCGTTGAGGAGTTAGAACGTCATTTTCAGATGAGTTGTG
AGTCACTGAACCCTATGTCTTTGTTTGAGAATAAGGTGGCCCAGAAACGCCGATCGTCACTGAATTGTCAGCATTTTGGT
ATTGCGGCGGGGATGGCAATGCGTGGTCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-329)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U1X938

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.392

99.413

0.531
  pilM Vibrio campbellii strain DS40M4

47.076

100

0.472